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Findings from VIT University Provides New Data on Microbiology and Biotechnology (Biosynthesis and hyper production of pullulan by a newly isolated...

August 6, 2014



Findings from VIT University Provides New Data on Microbiology and Biotechnology (Biosynthesis and hyper production of pullulan by a newly isolated strain of Aspergillus japonicus-VIT-SB1)

By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biotechnology. According to news reporting originating in Tamil Nadu, India, by NewsRx journalists, research stated, "The main focus of this study was to screen and characterize novel microbial strains isolated from culinary leaf samples, capable of producing high concentrations of pullulan. Hundred isolates were screened from the phylloplane of different plants."

The news reporters obtained a quote from the research from VIT University, "The results revealed that eight strains had the capability to produce exopolysaccharide (EPS) and only one potential strain (designated as VIT-SB1) could produce the significant amount of EPS (3.9 +/- A 0.02 %) on the 6th day of the fermentation without optimisation. The EPS synthesized by VIT-SB1 strain was confirmed to be pullulan on the basis of the results of FT-IR, HPLC and the enzymatic (Pullulanase) analysis. More than 91 % hydrolysis of pullulan by pullulanase enzyme also indicated the presence of alpha (1 ? 6) glycosidic linkages of alpha (1 ? 4) linked maltotriose units. This VIT-SB1 strain was identified as Aspergillus japonicus based on the nucleotide sequence of the D1/D2 domain of Large-Subunit rRNA gene. The sequence was submitted to the GenBank Nucleotide sequence database with Accession No: KC128815. This study has confirmed that pullulan production capacity of this novel strain and Aureobasidium pullulans are comparable."

According to the news reporters, the research concluded: "Hence Aspergillus japonicus-VIT-SB1 strain can be commercially exploited as a potential pullulan producing strain."

For more information on this research see: Biosynthesis and hyper production of pullulan by a newly isolated strain of Aspergillus japonicus-VIT-SB1. World Journal of Microbiology & Biotechnology, 2014;30(7):2045-2052. World Journal of Microbiology & Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA (see also Biotechnology).

Our news correspondents report that additional information may be obtained by contacting B. Mishra, VIT Univ, Sch Bio Sci & Technol, Vellore 632014, Tamil Nadu, India.

Keywords for this news article include: Asia, Biotechnology, India, Tamil Nadu

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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