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Data on Molecular Recognition Reported by Researchers at University of Notre Dame [Molecular Recognition of Methyl alpha-D-Mannopyranoside by...

August 5, 2014



Data on Molecular Recognition Reported by Researchers at University of Notre Dame [Molecular Recognition of Methyl alpha-D-Mannopyranoside by Antifreeze (Glyco)Proteins]

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Molecular Recognition. According to news reporting originating from Notre Dame, Indiana, by NewsRx correspondents, research stated, "Antifreeze proteins and glycoproteins [AF(G)Ps] have been well-known for more than three decades for their ability to inhibit the growth and recrystallization of ice through binding to specific ice crystal faces, and they show remarkable structural compatibility with specific ice crystal faces. Here, we show that the crystal growth faces of methyl alpha-D-mannopyranoside (MDM), a representative pyranose sugar, also show noteworthy structural compatibility with the known periodicities of AF(G)Ps."

Our news editors obtained a quote from the research from the University of Notre Dame, "We selected fish AFGPs (AFGP8, AFGP1-5), and a beetle AFP (DAFP1) with increasing antifreeze activity as potential additives for controlling MDM crystal growth. Similar to their effects on ice growth, the AF(G)Ps can inhibit MDM crystal growth and recrystallization, and more significantly, the effectiveness for the AF(G)Ps are well correlated with their antifreeze activity. MDM crystals grown in the presence of AF(G)Ps are smaller and have better defined shapes and are of higher quality as indicated by single crystal X-ray diffraction and polarized microscopy than control crystals, but no new polymorphs of MDM were identified by single crystal X-ray diffraction, solid-state NMR, and attenuated total reflectance infrared spectroscopy. The observed changes in the average sizes of the MDM crystals can be related to the changes in the number of the MDM nuclei in the presence of the AF(G)Ps. The critical free energy change differences of the MDM nucleation in the absence and presence of the additives were calculated. These values are close to those of the ice nucleation in the presence of AF(G)Ps suggesting similar interactions are involved in the molecular recognition of MDM by the AF(G)Ps. To our knowledge this is the first report where AF(G)Ps have been used to control crystal growth of carbohydrates and on AFGPs controlling non-ice-like crystals. Our finding suggests MDM might be a possible alternative to ice for studying the detailed mechanism of AF(G)P crystal interactions. The relationships between AF(G)Ps and carbohydrate binding proteins are also discussed."

According to the news editors, the research concluded: "The structural compatibility between AF(G)Ps and growing crystal faces demonstrated herein adds to the repertoire of molecular recognition by AF(G)Ps, which may have potential applications in the sugar, food, pharmaceutical, and materials industries."

For more information on this research see: Molecular Recognition of Methyl alpha-D-Mannopyranoside by Antifreeze (Glyco)Proteins. Journal of the American Chemical Society, 2014;136(25):8973-8981. Journal of the American Chemical Society can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)

The news editors report that additional information may be obtained by contacting S. Wang, University of Notre Dame, Dept. of Biol Sci, Notre Dame, IN 46556, United States. Additional authors for this research include X. Wen, A.L. DeVries, Y. Bagdagulyan, A. Morita, J.A. Golen, J.G. Duman and A.L. Rheingold (see also Molecular Recognition).

Keywords for this news article include: Indiana, Notre Dame, United States, Nanotechnology, Emerging Technologies, Molecular Recognition, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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