Data on Biological and Medical Science Detailed by Researchers at University of Edinburgh (Probing label-free intracellular quantification of free peptide by MALDI-ToF mass spectrometry)
By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Health and Medicine. According to news reporting originating in Edinburgh, United Kingdom, by NewsRx journalists, research stated, "Cell-penetrating peptides are promising reagents for gene and drug delivery. They can efficiently traverse the plasma membrane and deliver various cargo materials ranging from genes to nanoparticles."
The news reporters obtained a quote from the research from the University of Edinburgh, "The functional efficiency of cargo often depends on the completeness of intracellular peptide uptake, which can be measured, but its quantification remains largely inconclusive. Existing approaches rely on the use of radioactive and fluorescent labels or tags which allow colorimetric, fluorescent or spectrometric detection, but lack the ability to detect free peptide. Herein we describe a generic label- and tag-free method to measure the concentration of internalised peptide by matrix-assisted laser desorption/ionisation time of flight mass spectrometry. Quantification is preceded by two-dimensional chromatography and is performed at benign temperatures for the lysates of human dermal fibroblasts transfected with cell penetrating peptides in free form. Isotopically labelled peptides of the same structure are used as internal standards to enable accurate determination of concentration of the recovered free peptide."
According to the news reporters, the research concluded: "The method offers a minimalistic approach for intracellular quantification, which can be used as a correlative measure for fluorescence-based imaging methods."
For more information on this research see: Probing label-free intracellular quantification of free peptide by MALDI-ToF mass spectrometry. Methods, 2014;68(2):331-337. Methods can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Methods - www.elsevier.com/wps/product/cws_home/622914)
Our news correspondents report that additional information may be obtained by contacting P.D. Rakowska, University of Edinburgh, Sch Phys & Astron, Edinburgh EH9 3JZ, Midlothian, United Kingdom. Additional authors for this research include B. Lamarre and M.G. Ryadnov (see also Health and Medicine).
Keywords for this news article include: Europe, Edinburgh, United Kingdom, Health and Medicine
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