Report Summarizes Analytical Science Study Findings from Wuhan University [Laminar flow mediated continuous single-cell analysis on a novel poly(dimethylsiloxane) microfluidic chip]
By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Science. According to news originating from Wuhan, People's Republic of China, by NewsRx correspondents, research stated, "A novel microfluidic chip with simple design, easy fabrication and low cost, coupled with high-sensitive laser induced fluorescence detection, was developed to provide continuous single-cell analysis based on dynamic cell manipulation in flowing streams. Making use of laminar flows, which formed in microchannels, single cells were aligned and continuously introduced into the sample channel and then detection channel in the chip."
Our news journalists obtained a quote from the research from Wuhan University, "In order to rapidly lyse the moving cells and completely transport cellular contents into the detection channel, the angle of the side-flow channels, the asymmetric design of the channels, and the number, shape and layout of micro-obstacles were optimized for effectively redistributing and mixing the laminar flows of single cells suspension, cell lysing reagent and detection buffer. The optimized microfluidic chip was an asymmetric structure of three microchannels, with three microcylinders at the proper positions in the intersections of channels. The microchip was evaluated by detection of anticancer drug doxorubicin (DOX) uptake and membrane surface P-glycoprotein (P-gp) expression in single leukemia K562 cells. An average throughput of 6-8 cells min(-1) was achieved. The detection results showed the cellular heterogeneity in DOX uptake and surface P-gp expression within K562 cells."
According to the news editors, the research concluded: "Our researches demonstrated the feasibility and simplicity of the newly developed microfluidic chip for chemical single-cell analysis."
For more information on this research see: Laminar flow mediated continuous single-cell analysis on a novel poly(dimethylsiloxane) microfluidic chip. Analytica Chimica Acta, 2014;820():104-11. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news correspondents report that additional information may be obtained from B. Deng, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education) and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, People's Republic of China. Additional authors for this research include Y. Tian, X. Yu, J. Song, F. Guo, Y. Xiao and Z. Zhang (see also Science).
Keywords for this news article include: Asia, Wuhan, Science, People's Republic of China.
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