Investigators at Tokyo University of Agriculture and Technology Report Findings in Heat-Shock Proteins (Single-molecule detection of chaperonin dynamics through polarization rotation modulation of CdSe QD luminescence imaging)
By a News Reporter-Staff News Editor at Science Letter -- Investigators publish new report on Proteins. According to news originating from Tokyo, Japan, by NewsRx correspondents, research stated, "We report our recent trials examining the single-molecule three-dimensional (3D) detection of protein conformational dynamics at room temperature. Using molecular chaperones as model proteins and cadmium selenide (CdSe) semiconductor quantum dots (QDs) as nanometer-scale probes, we monitored the temporal evolution of ATP-induced conformation changes with a total internal reflection fluorescence (TIRF) microscopy imaging technique in buffer solutions."
Our news journalists obtained a quote from the research from the Tokyo University of Agriculture and Technology, "The two-dimensional (2D) degenerate nature of the emission dipoles of the QDs, due to the uniaxial wurtzite crystal structure, made it possible to capture the 3D orientation using a polarization modulation technique in real time. The temporal resolution was half the period of analyzer rotation."
According to the news editors, the research concluded: "Although still insufficient, the obtained signals suggest possible 3D detection of specific motions, which supports the two-step conformational changes triggered by ATP attachment."
For more information on this research see: Single-molecule detection of chaperonin dynamics through polarization rotation modulation of CdSe QD luminescence imaging. Journal of Luminescence, 2014;152():88-92. Journal of Luminescence can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Luminescence - www.elsevier.com/wps/product/cws_home/505700)
The news correspondents report that additional information may be obtained from T. Tani, Tokyo University of Agriculture & Technology, Inst Technol, Div Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan. Additional authors for this research include M. Oda, D. Araki, T. Miyashita, K. Nakajima, M. Arita and M. Yohda (see also Proteins).
Keywords for this news article include: Asia, Tokyo, Japan, Chaperonins, Heat-Shock Proteins
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