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Findings from Technical University Reveals New Findings on Chromatography (Fast carbohydrate analysis via liquid chromatography coupled with ultra...

August 1, 2014



Findings from Technical University Reveals New Findings on Chromatography (Fast carbohydrate analysis via liquid chromatography coupled with ultra violet and electrospray ionization ion trap detection in 96-well format)

By a News Reporter-Staff News Editor at Science Letter -- Current study results on Science have been published. According to news reporting out of Munich, Germany, by NewsRx editors, research stated, "A fast carbohydrate screening platform processible in 96-well format is described. The method is suitable for the determination of various carbohydrates out of complex mixtures as obtained by acidic hydrolysis of carbohydrates polymers."

Our news journalists obtained a quote from the research from Technical University, "The chromatographic conditions for an efficient separation (12 min) and the derivatization process with 1-phenyl-3-methyl-5-pyrazolone (PMP) were optimized for high resolution separation and simultaneous determination of deoxy-, amino-, anhydro-sugars as well as hexoses, pentoses, dimers, uronic acids and degradation products like furfural and hydroxymethylfurfural (HMF). The potential to quantify with UV- and MS-detector in the same range has been demonstrated for 20 different compounds. Finally, the matrix effects of the hydrolysis were positively evaluated. The micro scale hydrolysis and PMP-derivatization without any extraction or drying steps, both in 96-well format, result in a fast and intuitive sample preparation."

According to the news editors, the research concluded: "In combination with a fast liquid chromatography coupled to UV and electrospray ionization ion trap detection (LC-UV-ESI-MS/MS) for the qualification and quantification of various sugars, dimers and degradation products, this method shows great performance in carbohydrate analysis. ."

For more information on this research see: Fast carbohydrate analysis via liquid chromatography coupled with ultra violet and electrospray ionization ion trap detection in 96-well format. Journal of Chromatography A, 2014;1350():44-50. Journal of Chromatography A can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Journal of Chromatography A - www.elsevier.com/wps/product/cws_home/502688)

Our news journalists report that additional information may be obtained by contacting B. Ruhmann, Technical University of Munich, D-80290 Munich, Germany. Additional authors for this research include J. Schmid and V. Sieber (see also Science).

Keywords for this news article include: Munich, Germany, Europe, Science

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Science Letter


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