Study Results from Changzhou University Broaden Understanding of Capillary Electrophoresis (Studies on multivalent interactions of quantum dots-protein self-assemble using fluorescence coupled capillary electrophoresis)
By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Capillary Electrophoresis is the subject of a report. According to news reporting from Changzhou, People's Republic of China, by NewsRx journalists, research stated, "Nanoparticle-biomolecules self-assembly is the key to the understanding of biomolecular coating of nanoparticle. However, the self-assembly of biomolecules with nanoparticles is still under-exploited because of the lack of an efficient method to detect the subtle changes in the surface of nanoparticles."
The news correspondents obtained a quote from the research from Changzhou University, "In this study, we utilized fluorescence coupled capillary electrophoresis (CE-FL) to probe the binding interaction between a multivalent ligand (dBSA, denatured bovine serum albumin which contains multiple thiol groups) and CdSe/ZnS quantum dots (QDs, 5 nm in diameter). The yield of QDs-dBSA complex increased with increasing molar ratio of dBSA to QDs, which plateaued at a ratio of 8:1. Besides, QDs-dBSA complex showed good stability due to the multivalent interaction, revealing that dBSA is a superior ligand for QDs. The self-assembly kinetics of QDs with dBSA manifested a bi-phasic kinetics with a linear initial stage followed by a saturating stage. This work revealed for the first time that there exist two types of binding sites on the surface of QDs for dBSA: one type termed 'high priority' binding sites, which preferentially bind to the protein, whereas the 'low priority' sites are occupied only after the first-type binding sites are fully bound. Our work thereby represents the first example of systematic investigation on the details of the metal-affinity driven self-assembly between QDs and dBSA utilizing the high-resolution CE-FL."
According to the news reporters, the research concluded: "It also expanded the application of CE-FL in the study of nanoparticle-biomolecule interaction and kinetics analysis."
For more information on this research see: Studies on multivalent interactions of quantum dots-protein self-assemble using fluorescence coupled capillary electrophoresis. Journal of Nanoparticle Research, 2014;16(7):1-7. Journal of Nanoparticle Research can be contacted at: Springer, Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands. (Springer - www.springer.com; Journal of Nanoparticle Research - www.springerlink.com/content/1388-0764/)
Our news journalists report that additional information may be obtained by contacting J.H. Wang, Changzhou Univ, Inst Biomed Engn & Hlth Sci, Changzhou 213164, Jiangsu, People's Republic of China. Additional authors for this research include J.Y. Li, Y.W. Teng, W. Hu, H. Chai, J.C. Li, C.L. Wang, L. Qiu and P.J. Jiang (see also Capillary Electrophoresis).
Keywords for this news article include: Changzhou, People's Republic of China, Asia, Capillary Electrophoresis, Emerging Technologies, Nanoparticle, Nanotechnology, Quantum Dots, Quantum Physics
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