Study Findings from Chalmers University of Technology Broaden Understanding of Enzymes and Coenzymes (Single lipid vesicle assay for characterizing single-enzyme kinetics of phospholipid hydrolysis in a complex biological fluid)
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Enzymes and Coenzymes. According to news reporting from Gothenburg, Sweden, by NewsRx journalists, research stated, "Imaging of individual lipid vesicles is used to track single-enzyme kinetics of phospholipid hydrolysis. The method is employed to quantify the catalytic activity of phospholipase A2 (PLA2) in both pure and complex biological fluids."
The news correspondents obtained a quote from the research from the Chalmers University of Technology, "The measurements are demonstrated to offer a subpicomolar limit of detection (LOD) of human secretory PLA2 (sPLA2) in up to 1000-fold-diluted cerebrospinal fluid (CSF). An additional new feature provided by the single-enzyme sensitivity is that information about both relative concentration variations of active sPLA2 in CSF and the specific enzymatic activity can be simultaneously obtained. When CSF samples from healthy controls and individuals diagnosed with Alzheimer's disease (AD) are analyzed, the specific enzymatic activity is found to be preserved within 7% in the different CSF samples whereas the enzyme concentration differs by up to 56%. This suggests that the previously reported difference in PLA2 activity in CSF samples from healthy and AD individuals originates from differences in the PLA2 expression level rather than from the enzyme activity. Conventional ensemble averaging methods used to probe sPLA2 activity do not allow one to obtain such information."
According to the news reporters, the research concluded: "Together with an improvement in the LOD of at least 1 order of magnitude compared to that of conventional assays, this suggests that the method will become useful in furthering our understanding of the role of PLA2 in health and disease and in detecting the pharmacodynamic effects of PLA2-targeting drug candidates."
For more information on this research see: Single lipid vesicle assay for characterizing single-enzyme kinetics of phospholipid hydrolysis in a complex biological fluid. Journal of the American Chemical Society, 2013;135(38):14151-8. (American Chemical Society - www.acs.org; Journal of the American Chemical Society - www.pubs.acs.org/journal/jacsat)
Our news journalists report that additional information may be obtained by contacting S.R. Tabaei, Dept. of Applied Physics, Chalmers University of Technology , Gothenburg, Sweden. Additional authors for this research include M. Rabe, H. Zetterberg, V.P. Zhdanov and F. Hook (see also Enzymes and Coenzymes).
Keywords for this news article include: Gothenburg, Sweden, Europe, Enzymes and Coenzymes.
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