Studies from State University Yield New Information about Biological Pigments (Methodology for Porphyrin Isolation by High-Performance Countercurrent Chromatography)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Biological Factors have been published. According to news reporting originating in Rio de Janeiro, Brazil, by NewsRx journalists, research stated, "Countercurrent chromatography is a versatile technique for the isolation of a wide variety of plant substances. However, little attention has been devoted to the application of this technique for the isolation of porphyrins."
The news reporters obtained a quote from the research from State University, "This class of compounds are of great importance in the medical area and in photocatalysis due to their heterocyclic structure, composed of four modified pyrrol subunits interconnected on their a carbon atoms by methinic bridges. The methanol extract of Gallesia integrifolia was partitioned using different solvents; the dichloromethane fraction was then submitted to countercurrent chromatography. The solvent system composed of n-hexane, ethyl acetate, methanol, and water (1:2.5:2.5:1) was chosen to perform the chromatographic analysis due to the enhanced solubility and the best distribution coefficients of the target compounds. Two porphyrins were isolated by this method and identified as 13(2)-hydroxypheophorbide a methyl ester and pheophorbide a, methyl ester."
According to the news reporters, the research concluded: "The solvent system proposed provided good distribution coefficients for both substances (1.27 and 1.87, respectively), and a high resolution factor."
For more information on this research see: Methodology for Porphyrin Isolation by High-Performance Countercurrent Chromatography. Natural Product Communications, 2014;9(6):841-842. Natural Product Communications can be contacted at: Natural Products Inc, 7963 Anderson Park Ln, Westerville, OH 43081, USA (see also Biological Factors).
Our news correspondents report that additional information may be obtained by contacting A.C. Ramos, Univ Estadual Norte Fluminense, Lab Ciencias Quim, Center Ciencia & Tecnol, BR-28013602 Rio De Janeiro, Brazil. Additional authors for this research include F.S. Neves, M.R.G. Vega, E.J. Maria and R.R. Oliveira.
Keywords for this news article include: Rio de Janeiro, Brazil, South America, Biological Factors, Biological Pigments, Porphyrins
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