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Studies from Pomeranian Medical University in the Area of Cancer Gene Therapy Reported (Cell Type Related Differences in Staining with Pentameric...

July 28, 2014

Studies from Pomeranian Medical University in the Area of Cancer Gene Therapy Reported (Cell Type Related Differences in Staining with Pentameric Thiophene Derivatives)

By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- A new study on Biotechnology is now available. According to news originating from Szczecin, Poland, by NewsRx correspondents, research stated, "Fluorescent compounds capable of staining cells selectively without affecting their viability are gaining importance in biology and medicine. Recently, a new family of optical dyes, denoted luminescent conjugated oligothiophenes (LCOs), has emerged as an interesting class of highly emissive molecules for studying various biological phenomena."

Our news journalists obtained a quote from the research from Pomeranian Medical University, "Properly functionalized LCOs have been utilized for selective identification of disease-associated protein aggregates and for selective detection of distinct cells. Herein, we present data on differential staining of various cell types, including cancer cells. The differential staining observed with newly developed pentameric LCOs is attributed to distinct side chain functionalities along the thiophene backbone. Employing flow cytometry and fluorescence microscopy we examined a library of LCOs for stainability of a variety of cell lines. Among tested dyes we found promising candidates that showed strong or moderate capability to stain cells to different extent, depending on target cells. Hence, LCOs with diverse imidazole motifs along the thiophene backbone were identified as an interesting class of agents for staining of cancer cells, whereas LCOs with other amino acid side chains along the backbone showed a complete lack of staining for the cells included in the study. Furthermore, for p-HTMI,a LCO functionalized with methylated imidazole moieties, the staining was dependent on the p53 status of the cells, indicating that the molecular target for the dye is a cellular component regulated by p53."

According to the news editors, the research concluded: "We foresee that functionalized LCOs will serve as a new class of optical ligands for fluorescent classification of cells and expand the toolbox of reagents for fluorescent live imaging of different cells."

For more information on this research see: Cell Type Related Differences in Staining with Pentameric Thiophene Derivatives. Cytometry Part A, 2014;85A(7):628-635. Cytometry Part A can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell -; Cytometry Part A -

The news correspondents report that additional information may be obtained from A. Cieslar-Pobuda, Pomeranian Medical University, Dept. of Pathol, Szczecin, Poland. Additional authors for this research include M. Back, K. Magnusson, M.V. Jain, M. Rafat, S. Ghavami, K.P.R. Nilsson and M.J. Los (see also Biotechnology).

Keywords for this news article include: Szczecin, Poland, Europe, Biotechnology, Cancer Gene Therapy, Cytometry, Genetics, Sulfur Compounds, Thiophenes, p53 Gene

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Source: Cancer Gene Therapy Week

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