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Researchers from LFB Biotechnology Report New Studies and Findings in the Area of Chromatography (LC-MS analysis of polyclonal IgGs using IdeS...

August 1, 2014



Researchers from LFB Biotechnology Report New Studies and Findings in the Area of Chromatography (LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring)

By a News Reporter-Staff News Editor at Health & Medicine Week -- Fresh data on Laboratory Techniques are presented in a new report. According to news reporting out of Les Ulis, France, by NewsRx editors, research stated, "Susceptibility of IgGs to oxidation is a significant issue for intravenous immunoglobulin preparations (IVIG) in liquid solution and raises both safety and efficacy concerns. Here we present an optimized chromatography method coupled to mass spectrometry (MS) to determine the oxidation of Fc/2 fragments derived from polyclonal IgGs after IdeS treatment."

Our news journalists obtained a quote from the research from LFB Biotechnology, "Separation of the four IgG subclasses was achieved using a diphenyl column and UV/MS detections were used for quantification and characterization. Several oxidized Fc/2 fragments generated by stress conditions were resolved and oxidized methionines were identified."

According to the news editors, the research concluded: "This procedure can be used to monitor the oxidative status of IVIG preparations during formulation or stability studies."

For more information on this research see: LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014;961():1-4. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Laboratory Techniques).

Our news journalists report that additional information may be obtained by contacting Y. Leblanc, LFB Biotechnol, Analyt Department, F-91958 Courtaboeuf, Les Ulis, France. Additional authors for this research include M. Romanin, N. Bihoreau and G. Chevreux.

Keywords for this news article include: Les Ulis, France, Europe, Laboratory Techniques

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Health & Medicine Week


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