Researchers at University of Michigan Report New Data on G-Protein-Coupled Receptors (Microfluidic interrogation and mathematical modeling of multi-regime calcium signaling dynamics)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Membrane Proteins. According to news reporting originating from Ann Arbor, Michigan, by NewsRx correspondents, research stated, "Through microfluidic interrogation we analyzed real-time calcium responses of HEK293 cells stimulated with short pulses of the M3 muscarinic receptor ligand carbachol in two different concentration regimes. Lower ligand concentrations elicit oscillatory calcium signals while higher concentrations trigger a rapid rise that eventually settles down at a steady-state slightly above pre-stimulus levels, referred to as an acute signal."
Our news editors obtained a quote from the research from the University of Michigan, "Cells were periodically pulsed with carbachol at these two concentration regimes using a custom-made microfluidic platform, and the resulting calcium signals were measured with a single fluorescent readout. Pulsed stimulations at these two concentration regimes resulted in multiple types of response patterns that each delivered complementary information about the M3 muscarinic receptor signaling pathway. These multiple types of calcium response patterns enabled development of a comprehensive mathematical model of multi-regime calcium signaling. The resulting model suggests that dephosphorylation of deactivated receptors is rate limiting for recovery of calcium signals in the acute regime (high ligand concentration), while calcium replenishment and IP3 production determine signal recovery in the oscillatory regime (low ligand concentration)."
According to the news editors, the research concluded: "This study not only provides mechanistic insight into multi-regime signaling of the M3 muscarinic receptor pathway, but also provides a general strategy for analyzing multi-regime pathways using only one fluorescent readout."
For more information on this research see: Microfluidic interrogation and mathematical modeling of multi-regime calcium signaling dynamics. Integrative Biology, 2013;5(7):932-9. (Royal Society of Chemistry - www.rsc.org/; Integrative Biology - pubs.rsc.org/en/journals/journalissues/ib)
The news editors report that additional information may be obtained by contacting A. Jovic, Biomedical Engineering Department, University of Michigan, Gerstacker Building, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, United States. Additional authors for this research include S.M. Wade, R.R. Neubig, J.J. Linderman and S. Takayama (see also Membrane Proteins).
Keywords for this news article include: Ann Arbor, Michigan, United States, North and Central America, Cholinergic Receptors, G Protein Coupled Receptors, Membrane Proteins, Muscarinic Receptors.
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