News Column

New Yersinia pestis Study Results from Pasteur Institute Described (Detection of Yersinia pestis in Environmental and Food Samples by Intact Cell...

July 29, 2014



New Yersinia pestis Study Results from Pasteur Institute Described (Detection of Yersinia pestis in Environmental and Food Samples by Intact Cell lmmunocapture and Liquid Chromatography-Tandem Mass Spectrometry)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteobacteria. According to news reporting originating in Paris, France, by NewsRx journalists, research stated, "Yersinia pestis is the causative agent of bubonic and pneumonic plague, an acute and often fatal disease in humans. In addition to the risk of natural exposure to plague, there is also the threat of a bioterrorist act, leading to the deliberate spread of the bacteria in the environment or food."

The news reporters obtained a quote from the research from Pasteur Institute, "We report here an immuno-liquid chromatography tandem mass spectrometry (immuno-LC MS/MS) method for the direct (i.e., without prior culture), sensitive, and specific detection of Y. pestis in such complex samples. In the first step, a bottom-up proteomics approach highlighted three relevant protein markers encoded by the Y. pestis-specific plasmids pFra (murine toxin) and pPla (plasminogen activator and pesticin). Suitable proteotypic peptides were thoroughly selected to monitor the three protein markers by targeted MS using the selected reaction monitoring (SRM) mode. Immunocapture conditions were optimized for the isolation and concentration of intact bacterial cells from complex samples. The immuno-LC SRM assay has a limit of detection of 2 X 10(4) CFU/mL in milk or tap water, which compares well with those of state-of-the-art immunoassays."

According to the news reporters, the research concluded: "Moreover, we report the first direct detection of Y pestis in soil, which could be extremely useful in confirming Y. pestis persistence in the ground."

For more information on this research see: Detection of Yersinia pestis in Environmental and Food Samples by Intact Cell lmmunocapture and Liquid Chromatography-Tandem Mass Spectrometry. Analytical Chemistry, 2014;86(12):6144-6152. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)

Our news correspondents report that additional information may be obtained by contacting J. Chenau, Inst Pasteur, Unite Rech Yersinia, F-75724 Paris 15, France. Additional authors for this research include F. Fenaille, S. Simon, S. Filali, H. Volland, C. Junot, E. Carniel and F. Becher (see also Proteobacteria).

Keywords for this news article include: Paris, France, Europe, Enterobacteriaceae, Gram-Negative Bacteria, Proteobacteria, Yersinia pestis

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


For more stories covering the world of technology, please see HispanicBusiness' Tech Channel



Source: Life Science Weekly


Story Tools






HispanicBusiness.com Facebook Linkedin Twitter RSS Feed Email Alerts & Newsletters