New Findings Reported from Huazhong Agricultural University Describe Advances in Pseudorabies (Stretch-Stowage-Growth Strategy to Fabricate Tunable Triply-Amplified Electrochemiluminescence Immunosensor for Ultrasensitive Detection of ...)
By a News Reporter-Staff News Editor at Biotech Week -- A new study on Virus Diseases is now available. According to news reporting from Wuhan, People's Republic of China, by NewsRx journalists, research stated, "Triply amplified electrochemical biosensors have attracted particular attention in the detection of low-abundance biomarkers. The universal construction routes for nonenzymatic triply amplified and even multiply amplified biosensors are extremely desirable but remain challenging."
The news correspondents obtained a quote from the research from Huazhong Agricultural University, "Here, we proposed a 'stretch-stowage-growth' strategy to tunably fabricate a nonenzymatic triply amplified or multiply amplified electrochemiluminescence (ECL) immunosensor for ultrasensitive determining pseudorabies virus (PrV) antibody. Based on the matrix role of gold nanoparticle-graphene nanosheet (Au-GN) hybrids, carrier role of silicon nanoparticles (SNPs) and bridge role of 'biotin-streptavidin-biotin' (B-SA-B) structure, the establishment processes were defined as 'stretch', 'stowage', and 'growth', respectively. Relying on the interaction of antigen-antibody and of B-SA, the 'Au-GN/PrV (Ag)/PrV antibody (Abi)/biotinylated IgG (B-Ab(2))/SA/biotinylated Ru(bpy)(3)(2+)-encapsulated SNPs (B-Ru@SNPs)' triply amplified biosensor could be fabricated and exhibited better analytical performance not only toward monoclonal PrV antibody with a linear detection range from 50 ng mL(-1) to 1 pg mL(-1) and a detection limit of 0.40 pg mL(-1), but also toward actual serum samples when compared with enzyme-linked immunosorbent assay and fluorometry. Furthermore, multiply amplified biosensors could be conveniently fabricated by controllably repeating the combination of B-Ru@SNPs and SA to form the B-SA-B structure. After it was repeated three times, the multiply amplified biosensor stretched to the maximum of signal amplification and achieved a luminescence quantum efficiency about 23.1-fold higher than the triply amplified biosensor."
According to the news reporters, the research concluded: "The tunable biosensor exhibits good stability, acceptable reproducibility and accuracy, suggesting its potential applications in clinical diagnostics."
For more information on this research see: Stretch-Stowage-Growth Strategy to Fabricate Tunable Triply-Amplified Electrochemiluminescence Immunosensor for Ultrasensitive Detection of Pseudorabies Virus Antibody. Analytical Chemistry, 2014;86(12):5749-5757. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news journalists report that additional information may be obtained by contacting K. Shao, Huazhong Agricultural University, Coll Sci, State Key Lab Agr Microbiol, Wuhan 430070, People's Republic of China. Additional authors for this research include J. Wang, X.C. Jiang, F. Shao, T.T. Li, S.Y. Ye, L. Chen and H.Y. Han (see also Virus Diseases).
Keywords for this news article include: Wuhan, People's Republic of China, Asia, Antibodies, Bioengineering, Bionanotechnology, Biosensing, Biotechnology, Blood Proteins, Central Nervous System Diseases, Central Nervous System Infections, Central Nervous System Viral Diseases, DNA Virus Infections, DNA Viruses, Herpesviridae Infections, Immunoglobulins, Immunology, Nanobiotechnology, Nanotechnology, Pseudorabies, Virus Diseases
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