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New Findings from P. Srilohasin and Co-Authors in the Area of Genetics and Tuberculosis Reported (Novel DNA Chip Based on a Modified DigiTag2 Assay...

August 1, 2014



New Findings from P. Srilohasin and Co-Authors in the Area of Genetics and Tuberculosis Reported (Novel DNA Chip Based on a Modified DigiTag2 Assay for High-Throughput Species Identification and Genotyping of Mycobacterium tuberculosis Complex ...)

By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- New research on Mycobacterium Infections is the subject of a report. According to news originating from Pathum Thani, Thailand, by NewsRx correspondents, research stated, "A multipurpose high-throughput genotyping tool for the assessment of recent epidemiological data and evolutional pattern in Mycobacterium tuberculosis complex (MTBC) clinical isolates was developed in this study. To facilitate processing, 51 highly informative single nucleotide polymorphisms (SNPs) were selected for discriminating the clinically most relevant MTBC species and genotyping M. tuberculosis into its principle genetic groups (PGGs) and SNP cluster groups (SCGs)."

Our news journalists obtained a quote from the research, "Because of the high flexibility of the DigiTag2 assay, the identical protocol and DNA array containing the identical set of probes were applied to the highly GC-rich mycobacterial genome. The specific primers with multiplex amplification and hybridization conditions based on the DigiTag2 principle were optimized and evaluated with 14 MTBC reference strains, 4 nontuberculous mycobacteria (NTM) isolates, and 322 characterized M. tuberculosis clinical isolates. The DNA chip that was developed revealed a 99.85% call rate, a 100% conversion rate, and 99.75% reproducibility. For the accuracy rate, 98.94% of positive calls were consistent with previous molecular characterizations. Our cost-effective technology was capable of simultaneously identifying the MTBC species and the genotypes of 96 M. tuberculosis clinical isolates within 6 h using only simple instruments, such as a thermal cycler, a hybridization oven, and a DNA chip scanner, and less technician skill was required than for other techniques."

According to the news editors, the research concluded: "We demonstrate this approach's potential as a simple, flexible, and rapid tool for providing clearer information regarding circulating MTBC isolates."

For more information on this research see: Novel DNA Chip Based on a Modified DigiTag2 Assay for High-Throughput Species Identification and Genotyping of Mycobacterium tuberculosis Complex Isolates. Journal of Clinical Microbiology, 2014;52(6):1962-1968. Journal of Clinical Microbiology can be contacted at: Amer Soc Microbiology, 1752 N St NW, Washington, DC 20036-2904, USA (see also Mycobacterium Infections).

The news correspondents report that additional information may be obtained from P. Srilohasin, Natl Sci & Technol Dev Agcy, Natl Center Genet Engn & Biotechnol, Minist Sci & Technol, Pathum Thani, Thailand. Additional authors for this research include A. Chaiprasert, K. Tokunaga, N. Nao and T. Prammananan.

Keywords for this news article include: Pathum Thani, Thailand, Asia, Actinobacteria, Actinomycetales, DNA Research, Genetics, Gram-Positive Asporogenous Rods, Gram-Positive Bacteria, Gram-Positive Rods, Mycobacteriaceae, Mycobacterium Infections, Mycobacterium Tuberculosis, Mycobacterium tuberculosis

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Genomics & Genetics Weekly


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