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New Biomedicine and Biomedical Engineering Study Findings Have Been Reported by P.F. Favreau and Colleagues (Excitation-scanning hyperspectral...

July 30, 2014



New Biomedicine and Biomedical Engineering Study Findings Have Been Reported by P.F. Favreau and Colleagues (Excitation-scanning hyperspectral imaging microscope)

By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Biotechnology. According to news reporting originating in Rochester, New York, by NewsRx journalists, research stated, "Hyperspectral imaging is a versatile tool that has recently been applied to a variety of biomedical applications, notably live-cell and whole-tissue signaling. Traditional hyperspectral imaging approaches filter the fluorescence emission over a broad wavelength range while exciting at a single band."

The news reporters obtained a quote from the research, "However, these emission-scanning approaches have shown reduced sensitivity due to light attenuation from spectral filtering. Consequently, emission scanning has limited applicability for time-sensitive studies and photosensitive applications. In this work, we have developed an excitation-scanning hyperspectral imaging microscope that overcomes these limitations by providing high transmission with short acquisition times. This is achieved by filtering the fluorescence excitation rather than the emission. We tested the efficacy of the excitation-scanning microscope in a side-by-side comparison with emission scanning for detection of green fluorescent protein (GFP)-expressing endothelial cells in highly autofluorescent lung tissue. Excitation scanning provided higher signal-to-noise characteristics, as well as shorter acquisition times (300 ms/wavelength band with excitation scanning versus 3 s/wavelength band with emission scanning). Excitation scanning also provided higher delineation of nuclear and cell borders, and increased identification of GFP regions in highly autofluorescent tissue."

According to the news reporters, the research concluded: "These results demonstrate excitation scanning has utility in a wide range of time-dependent and photosensitive applications."

For more information on this research see: Excitation-scanning hyperspectral imaging microscope. Journal of Biomedical Optics, 2014;19(4):139-148. Journal of Biomedical Optics can be contacted at: Spie-Soc Photo-Optical Instrumentation Engineers, 1000 20TH St, PO Box 10, Bellingham, WA 98225, USA (see also Biotechnology).

Our news correspondents report that additional information may be obtained by contacting P.F. Favreau, Semrock Inc, Rochester, NY 14624, United States. Additional authors for this research include C. Hernandez, T. Heaster, D.F. Alvarez, T.C. Rich, P. Prabhat and S.J. Leavesley.

Keywords for this news article include: Rochester, New York, United States, North and Central America, Biotechnology

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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