Findings on Antibiotics Detailed by Investigators at University of Regensburg (Determination of free vancomycin, ceftriaxone, cefazolin and ertapenem in plasma by ultrafiltration: Impact of experimental conditions)
By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Drugs and Therapies have been published. According to news originating from Regensburg, Germany, by NewsRx correspondents, research stated, "Ultrafiltration is a rapid and convenient method to determine the free concentrations of drugs. In the present work, we aimed to develop an ultrafiltration method which is appropriate for routine determination of the free fraction of vancomycin and highly protein bound beta-lactams such as ertapenem, ceftriaxone and cefazolin in plasma from intensive care unit patients."
Our news journalists obtained a quote from the research from the University of Regensburg, "Different filter types and experimental conditions (molecular weight cut-off, centrifugal force and time, pH, temperature) were evaluated and found to have influence on the result. In the final protocol, serum or plasma was buffered to pH 7.4-7.5, ultrafiltered at 1000 x g at 37 degrees C for 20 min using Nanosep Omega 10 K filters and subsequently analysed for the antibiotics by RP-HPLC with UV detection. The data from our investigation suggest to aim physiological conditions, i.e. 37 degrees C and pH 7.4, and low to moderate relative centrifugal forces in order to get reliable results."
According to the news editors, the research concluded: "With regard to the chromatographic separation, modulation of the pH in the range of 2.5-7.0 allows to determine several beta-lactams isocratically and/or to avoid interferences by co-administered drugs."
For more information on this research see: Determination of free vancomycin, ceftriaxone, cefazolin and ertapenem in plasma by ultrafiltration: Impact of experimental conditions. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014;961():97-102. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands (see also Drugs and Therapies).
The news correspondents report that additional information may be obtained from A. Kratzer, University of Regensburg, Dept. of Pharmacol, D-93053 Regensburg, Germany. Additional authors for this research include U. Liebchen, M. Schleibinger, M.G. Kees and F. Kees.
Keywords for this news article include: Regensburg, Germany, Europe, Antiinfectives, Cefotaxime, Ceftriaxone, Cephalosporins, Drugs, Drugs and Therapies, Glycopeptide Antibiotics, Glycopeptides, Sulfur Compounds, Therapy, Thiazines, Vancomycin
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