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Findings from University of California in the Area of Chemotherapy Described (Rapid assessment of drug response in cancer cells using microwell array...

July 30, 2014



Findings from University of California in the Area of Chemotherapy Described (Rapid assessment of drug response in cancer cells using microwell array and molecular imaging)

By a News Reporter-Staff News Editor at Biotech Week -- A new study on Drugs and Therapies is now available. According to news originating from Davis, California, by NewsRx correspondents, research stated, "Selection of personalized chemotherapy regimen for individual patients has significant potential to improve chemotherapy efficacy and to reduce the deleterious effects of ineffective chemotherapy drugs. In this study, a rapid and high-throughput in vitro drug response assay was developed using a combination of microwell array and molecular imaging."

Our news journalists obtained a quote from the research from the University of California, "The microwell array provided high-throughput analysis of drug response, which was quantified based on the reduction in intracellular uptake (2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose) (2-NBDG). Using this synergistic approach, the drug response measurement was completed within 4 h, and only a couple thousand cells were needed for quantification. The broader application of this microwell molecular imaging approach was demonstrated by evaluating the drug response of two cancer cell lines, cervical (HeLa) and bladder (5637) cancer cells, to two distinct classes of chemotherapy drugs (cisplatin and paclitaxel). This approach did not require an extended cell culturing period, and the quantification of cellular drug response was 4-16 times faster compared with other cell-microarray drug response studies. Moreover, this molecular imaging approach had comparable sensitivity to traditional cell viability assays, i.e., the MTT assay and propidium iodide labeling of cellular nuclei;and similar throughput results as flow cytometry using only 1,000-2,000 cells."

According to the news editors, the research concluded: "Given the simplicity and robustness of this microwell molecular imaging approach, it is anticipated that the assay can be adapted to quantify drug responses in a wide range of cancer cells and drugs and translated to clinical settings for a rapid in vitro drug response using clinically isolated samples."

For more information on this research see: Rapid assessment of drug response in cancer cells using microwell array and molecular imaging. Analytical and Bioanalytical Chemistry, 2014;406(17):4195-4206. Analytical and Bioanalytical Chemistry can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Analytical and Bioanalytical Chemistry - www.springerlink.com/content/1618-2642/)

The news correspondents report that additional information may be obtained from M.S. Wang, University of California, Dept. of Biol & Agr Engn, Davis, CA 95616, United States. Additional authors for this research include Z. Luo and N.T. Nitin (see also Drugs and Therapies).

Keywords for this news article include: Davis, California, United States, North and Central America, Cancer, Chemotherapy, Drugs, Drugs and Therapies, Emerging Technologies, Molecular Imaging, Nanotechnology, Oncology, Therapy

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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