Data on Nanoparticles Detailed by Researchers at National Tsing Hua University (Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Nanoparticles. According to news reporting out of Hsinchu, Taiwan, by NewsRx editors, research stated, "Developing methods that evaluate the cellular uptake of magnetic nanoparticles (MNPs) and nanotoxicity effects at single-cellular level are needed. In this study, magnetophoresis combining fluorescence based cytotoxicity assay was proposed to assess the viability and the single-cellular MNPs uptake simultaneously."
Our news journalists obtained a quote from the research from National Tsing Hua University, "Malignant cells (SKHep-1, HepG2, HeLa) were incubated with 10 nm anionic iron oxide nanoparticles. Prussian blue stain was performed to visualize the distribution of magnetic nanoparticles. MTT and fluorescence based assay analyzed the cytotoxicity effects of the bulk cell population and single cell, respectively. DAPI/PI stained was applied to evaluate death mechanism. The number of intracellular MNPs was found to be strongly correlated with the cell death. Significant differences between cellular MNP uptake in living and dead cells were observed."
According to the news editors, the research concluded: "The method could be useful for future study of the nanotoxicity induced by MNPs."
For more information on this research see: Compare analysis for the nanotoxicity effects of different amounts of endocytic iron oxide nanoparticles at single cell level. Plos One, 2014;9(5):e96550. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting C.Y. Huang, Dept. of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan. Additional authors for this research include T.R. Ger, Z.H. Wei and M.F Lai (see also Nanoparticles).
Keywords for this news article include: Hsinchu, Taiwan, Asia, Emerging Technologies, Nanoparticle, Nanoparticles, Nanotechnology.
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