Study Data from University of North Carolina Update Understanding of Antisense Technology [Evaluation of Tris[2-(Acryloyloxy) Ethyl]Isocyanurate Cross-Linked Polyethylenimine as Antisense Morpholino Oligomer Delivery Vehicle in Cell Culture and ...]
By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Biotechnology have been presented. According to news reporting originating from Charlotte, North Carolina, by NewsRx correspondents, research stated, "Hyperbranched poly(ester amine)s (PEAs) based on tris[2-(acryloyloxy)ethyl] isocyanurate (TAEI) cross-linked low-molecular-weight polyethylenimine (Mw: 0.8k/1.2k/2.0k) have been evaluated for delivering antisense phosphorodiamidate morpholino oligomer (PMO) in vitro and in vivo in the dystrophic mdx mouse. The results show that the PEAs constructed with polyethylenimine (PEI) 2.0k (C series) improved PMO delivery more efficiently than those constructed with PEI 0.8k (A series) or 1.2k (B series) in a GFP reporter-based C2C12 mouse myoblast culture system."
Our news editors obtained a quote from the research from the University of North Carolina, "The highest efficiency of exon-skipping in vitro with the PMO oligonucleotide targeting human dystrophin exon 50 was obtained when the PEA C12 [TAEI-PEI 2.0k (1: 2)] was used. Nearly all of the PEAs improved dystrophin expression in mdx mice by local injection with a 2-4-fold increase when compared with PMO alone."
According to the news editors, the research concluded: "Improved transfection efficiency and lower toxicity indicate the potential of the biodegradable PEA polymers as safe and efficient PMO delivery vectors for in vivo applications."
For more information on this research see: Evaluation of Tris[2-(Acryloyloxy) Ethyl]Isocyanurate Cross-Linked Polyethylenimine as Antisense Morpholino Oligomer Delivery Vehicle in Cell Culture and Dystrophic mdx Mice. Human Gene Therapy, 2014;25(5):419-427. Human Gene Therapy can be contacted at: Mary Ann Liebert, Inc, 140 Huguenot Street, 3RD Fl, New Rochelle, NY 10801, USA. (Mary Ann Liebert, Inc. - www.liebertpub.com; Human Gene Therapy - www.liebertpub.com/overview/human-gene-therapy-and-part-b-methods/19/)
The news editors report that additional information may be obtained by contacting M.X. Wang, University of North Carolina, Dept. of Biol, Charlotte, NC 28223, United States. Additional authors for this research include B. Wu, J.D. Tucker, P.J. Lu, C. Cloer and Q.L. Lu (see also Biotechnology).
Keywords for this news article include: Antisense Technology, Biotechnology, Charlotte, United States, North Carolina, Bioengineering, North and Central America
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