Study Data from Rockefeller University Update Knowledge of Organelles [Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP)]
By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators discuss new findings in Organelles. According to news reporting out of New York City, New York, by NewsRx editors, research stated, "Cellular diversity and architectural complexity create barriers to understanding the function of the mammalian CNS at a molecular level. To address this problem, we have recently developed a methodology that provides the ability to profile the entire translated mRNA complement of any genetically defined cell population."
Our news journalists obtained a quote from the research from Rockefeller University, "This methodology, which we termed translating ribosome affinity purification, or TRAP, combines cell type-specific transgene expression with affinity purification of translating ribosomes. TRAP can be used to study the cell type-specific mRNA profiles of any genetically defined cell type, and it has been used in organisms ranging from Drosophila melanogaster to mice and human cultured cells. Unlike other methodologies that rely on microdissection, cell panning or cell sorting, the TRAP methodology bypasses the need for tissue fixation or single-cell suspensions (and the potential artifacts that these treatments introduce) and reports on mRNAs in the entire cell body."
According to the news editors, the research concluded: "This protocol provides a step-by-step guide to implement the TRAP methodology, which takes 2 d to complete once all materials are in hand."
For more information on this research see: Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nature Protocols, 2014;9(6):1282-1291. Nature Protocols can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group - www.nature.com/; Nature Protocols - www.nature.com/nprot/)
Our news journalists report that additional information may be obtained by contacting M. Heiman, Rockefeller University, Howard Hughes Med Inst, Mol Biol Lab, New York, NY 10021, United States. Additional authors for this research include R. Kulicke, R.J. Fenster, P. Greengard and N. Heintz (see also Organelles).
Keywords for this news article include: Cytoplasm, Ribosomes, Organelles, New York City, United States, Cellular Structures, Intracellular Space, North and Central America
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