Study Data from Beijing University of Chemical Technology Update Understanding of Amino Acids (Precise measurement for the purity of amino acid and peptide using quantitative nuclear magnetic resonance)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Amino Acids have been published. According to news reporting originating in Beijing, People's Republic of China, by NewsRx journalists, research stated, "Precise measurement for the purity of organic compounds will fundamentally improve the capabilities and measurement services of the organic chemical analysis. Quantitative nuclear magnetic resonance (qNMR) is an important method to assess the purity of organic compounds."
The news reporters obtained a quote from the research from the Beijing University of Chemical Technology, "We presented a precise measurement method for the purity of small molecule with identification of impurities. In addition, the qNMR was rarely applied to purity of large compounds such as peptide, for which qNMR peaks are too crowded. Other than general idea of qNMR, we removed unwanted exchangeable peaks by proton exchange, as a new approach for qNMR, to make the quantitative protons of peptide isolated, which can ensure precise measurement. Moreover, a suitable internal standard, acesulfame potassium, was applied. The analytes were valine and peptide T5, due to their importance for protein analysis. For valine, the intraday CV was 0.052%, and the interday CV during 8 months was 0.071%. For peptide T5, simpler operation, shorter analytical time (1 h vs. 3 days) and smaller CV (0.36% vs. 0.93%) were achieved by qNMR, compared with a traditional method (amino acid based isotope labeled mass spectrometry) via a hydrolysis reaction."
According to the news reporters, the research concluded: "This method has greatly increased the quantitative precision of qNMR for small compounds, and extended application scope of qNMR from small compounds to peptides."
For more information on this research see: Precise measurement for the purity of amino acid and peptide using quantitative nuclear magnetic resonance. Talanta, 2014;125():94-101. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
Our news correspondents report that additional information may be obtained by contacting T. Huang, Beijing University of Chemical Technology, Coll Sci, Beijing 100029, People's Republic of China. Additional authors for this research include W. Zhang, X.H. Dai, X.G. Zhang, C. Quan, H.M. Li and Y. Yang (see also Amino Acids).
Keywords for this news article include: Asia, Beijing, Peptides, Proteins, Amino Acids, People's Republic of China
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