Studies from University of Rzeszow Describe New Findings in Hemeproteins (D-lactate-selective amperometric biosensor based on the cell debris of the recombinant yeast Hansenula polymorpha)
By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Proteins. According to news originating from Rzeszow, Poland, by NewsRx correspondents, research stated, "A D-lactate-selective biosensor has been developed using cells' debris of recombinant thermotolerant methylotrophic yeast Hansenula polymorpha, overproducing D-lactate: cytochrome c-oxidoreductase (EC 22.214.171.124, D-lactate dehydrogenase (cytochrome), DIDH). The H. polymorpha DIDH-producer was constructed in two steps. First, the gene CYB2 was deleted on the background of the C-105 (gcr1 catX) strain of H. polymorpha impaired in glucose repression and devoid of catalase activity to avoid specific L-lactate-cytochrome c oxidoreductase activity."
Our news journalists obtained a quote from the research from the University of Rzeszow, "Second, the homologous gene DLD1 coding for DIDH was overexpressed under the control of the strong H. polymorpha alcohol oxidase promoter in the frame of a plasmid for multicopy integration in the Delta cyb2 strain. The selected recombinant strain possesses 6-fold increased DIDH activity as compared to the initial strain. The cells' debris was used as a biorecognition element of a biosensor, since DIDH is strongly bound to mitochondrial membranes. The cells' debris, prepared by mechanic disintegration of recombinant cells, was immobilized on a graphite working electrode in an electrochemically generated layer using an Oscomplex modified cathodic electrodeposition polymer. Cytochrome c was used as additional native electron mediator to improve electron transfer from reduced DIDH to the working electrode."
According to the news editors, the research concluded: "The constructed D-lactate-selective biosensors are characterized by a high sensitivity (46.3-61.6 A M-1 m(-2)), high selectivity and sufficient storage stability."
For more information on this research see: D-lactate-selective amperometric biosensor based on the cell debris of the recombinant yeast Hansenula polymorpha. Talanta, 2014;125():227-232. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
The news correspondents report that additional information may be obtained from O.V. Smutok, Univ Rzeszow, Dept. of Biotechnol & Microbiol, PL-35601 Rzeszow, Poland. Additional authors for this research include K.V. Dmytruk, M.I. Karkovska, W. Schuhmann, M.V. Gonchar and A.A. Sibirny (see also Proteins).
Keywords for this news article include: Poland, Europe, Rzeszow, Biosensing, Cytochromes, Hemeproteins, Bioengineering, Bionanotechnology, Nanobiotechnology
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