Studies from Fuzhou University Provide New Data on DNA Research (Rolling circle amplification combined with gold nanoparticles-tag for ultra sensitive and specific quantification of DNA by inductively coupled plasma mass spectrometry)
By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on DNA Research are discussed in a new report. According to news reporting from Fujian, People's Republic of China, by NewsRx journalists, research stated, "A novel method for the ultra specific and sensitive detection of DNA in biological samples was described in this paper based on magnetic beads (MBs)-based rolling circle amplification combined with gold nano-particles (AuNPs)-aptamer labeling technique and inductively coupled plasma mass spectrometry (ICP-MS) detection. The proposed assay has an ultra-high sensitivity and stability, excellent specificity and more robust resistibility to the complex matrix due to the utilization of MBs-based rolling circle amplification, AuNPs-aptamer labeling technique and ICP-MS detection."
The news correspondents obtained a quote from the research from Fuzhou University, "It can be used to determine DNA or single nucleotide polymorphisms (SNP) with an extremely low detection limit of 0.1fM (1.0 x 10(-16)M) and a discrimination factor for single-base mismatch of 27. The proposed assay has been successfully used to detect DNA in serum sample with a recovery of 91-106% and a relative standard deviation (RSD)
According to the news reporters, the research concluded: "The ultra-high sensitivity and specificity, easiness of fabrication, operational convenience, short analysis time, better stability and robust resistibility to the complex matrix, make the assay a promising alternative for the detection of various DNA sequence in the clinical diagnosis."
For more information on this research see: Rolling circle amplification combined with gold nanoparticles-tag for ultra sensitive and specific quantification of DNA by inductively coupled plasma mass spectrometry. Biosensors & Bioelectronics, 2014;58():209-13. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting Y. He, Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Dept. of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, People's Republic of China. Additional authors for this research include D. Chen, M. Li, L. Fang, W. Yang, L. Xu and F. Fu (see also DNA Research).
Keywords for this news article include: Asia, Fujian, DNA Research, Nanoparticle, Nanotechnology, Emerging Technologies, People's Republic of China.
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