Studies from Chulalongkorn University Update Current Data on Leptospira (Development of an immunochromatographic test with anti-LipL32-coupled gold nanoparticles for Leptospira detection)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on Leptospira have been published. According to news originating from Bangkok, Thailand, by NewsRx correspondents, research stated, "Detection of antibody specific to Leptospira by various immunological techniques has been used for leptospirosis diagnosis. However, the sensitivity of antibody detection during the first few days after infection is low."
Our news journalists obtained a quote from the research from Chulalongkorn University, "Molecular techniques are suggested to provide earlier diagnosis than antibody detection, but a rapid and easy to perform assay for Leptospira antigen detection would provide an additional useful tool for disease diagnosis. In this study, we coupled gold nanoparticles with antibody to LipL32, a protein commonly found in pathogenic Leptospira. This coupled gold reagent was used in the immunochromatographic strip for Leptospira detection. We demonstrated that the sensitivity of Leptospira detection by this strip was 10(3) ml(-1). There was no positive result detected when strips were tested with non-pathogenic Leptospira, Staphylococcus aureus, Streptococcus group B, Acinetobacter baumannii, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Enterococcus faecalis or Enterococcus faecium."
According to the news editors, the research concluded: "These data suggest that gold nanoparticles coupled with antibody to LipL32 could be used for Leptospira detection by a rapid test based on an immunochromatographic technique."
For more information on this research see: Development of an immunochromatographic test with anti-LipL32-coupled gold nanoparticles for Leptospira detection. The New Microbiologica, 2014;37(2):201-7. The New Microbiologica can be contacted at: Luigi Ponzio E Figlio, Via D da Catalogna 1, 3, 27100 Pavia, Italy (see also Leptospira).
The news correspondents report that additional information may be obtained from C. Chirathaworn, Dept. of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Additional authors for this research include W. Janwitthayanan, A. Sereemaspun, K. Lertpocasombat, U. Rungpanich, P. Ekpo and D. Suwancharoen.
The publisher's contact information for the journal The New Microbiologica is: Luigi Ponzio E Figlio, Via D da Catalogna 1, 3, 27100 Pavia, Italy.
Keywords for this news article include: Asia, Antibodies, Bangkok, Thailand, Immunology, Nanoparticle, Blood Proteins, Leptospiraceae, Nanotechnology, Spirochaetales, Immunoglobulins, Emerging Technologies, Gram Negative Bacteria, Gram Negative Aerobic Rods and Cocci.
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