Studies from Chongqing Medical University in the Area of DNA Research Reported (DNA-based hybridization chain reaction for an ultrasensitive cancer marker EBNA-1 electrochemical immunosensor)
By a News Reporter-Staff News Editor at Cancer Weekly -- Current study results on DNA Research have been published. According to news reporting from Chongqing, People's Republic of China, by NewsRx journalists, research stated, "An ultrasensitive and selective electrochemical immunosensor was developed for the detection of Epstein Barr virus nuclear antigen 1 (EBNA-1). Firstly, a suspension of graphene sheets (GS) and multi-walled carbon nanotubes (MWCNTs) was prepared with the aid of chitosan (CS) solution and then modified on a glassy carbon electrode (GCE)."
The news correspondents obtained a quote from the research from Chongqing Medical University, "Gold nanoparticles (AuNPs) were then electrodeposited onto the surface of the GS-MWCNTs film by cyclic voltammetry (CV) to immobilize the captured antibodies. After that, specific sandwich immunoreactions were formed among the captured antibody, EBNA-1, and secondary antibody, DNA-coated carboxyl multi-wall carbon nanotubes (DNA-MWCNTs-Ab2). DNA initiator strands (S0) and secondary antibodies linked to the MWCNTs and double-helix DNA polymers were obtained by hybridization chain reaction (HCR), and here S0 on the MWCNTs propagates a chain reaction of hybridization events between two alternating hairpins to form a nicked double-helix. Finally, electroactive indicator doxorubicin hydrochloride was intercalated into the CG-GC steps between the HCR products and could produce an electrochemical signal, which was monitored by differential pulse voltammetry (DPV). Under optimum conditions, the amperometric signal increased linearly with the target concentrations (0.05-6.4ngmL(-1)), and the immunosensor exhibited a detection limit as low as 0.7pgmL(-1) (S/N=3). The proposed method showed acceptable stability and reproducibility, as well as favorable recovery for EBNA-1 in human serum."
According to the news reporters, the research concluded: "The proposed immunosensor provides a novel avenue for signal amplification and potential applications in bioanalysis and clinical diagnostics."
For more information on this research see: DNA-based hybridization chain reaction for an ultrasensitive cancer marker EBNA-1 electrochemical immunosensor. Biosensors & Bioelectronics, 2014;58():68-74. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting C. Song, Key Laboratory of Laboratory Medical Diagnostics of Education, Dept. of Laboratory Medicine, Chongqing Medical University, No 1 Yi Xue Yuan Road, Chongqing 400016, People's Republic of China. Additional authors for this research include G. Xie, L. Wang, L. Liu, G. Tian and H. Xiang (see also DNA Research).
Keywords for this news article include: Asia, Cancer, Nanotube, Oncology, Chongqing, Chemistry, Viral DNA, DNA Research, Nanotechnology, Electrochemical, Emerging Technologies, People's Republic of China.
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