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Studies from Biotechnology Research Institute Further Understanding of Transcription Factors (Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an...

July 8, 2014



Studies from Biotechnology Research Institute Further Understanding of Transcription Factors (Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44)

By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Proteins are discussed in a new report. According to news reporting originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Abscisic acid (ABA) signaling plays important roles in plant growth, development and adaptation to various stresses. RCAR1/PYL9 has been known as a cytoplasm and nuclear ABA receptor in Arabidopsis."

Our news editors obtained a quote from the research from Biotechnology Research Institute, "To obtain further insight into the regulatory mechanism of RCAR1/PYL9, a yeast two-hybrid approach was performed to screen for RCAR1/PYL9-interacting proteins and an R2R3-type MYB transcription factor, AtMYB44, was identified. The interaction between RCAR1/PYL9 and AtMYB44 was further confirmed by glutathione S-transferase (GST) pull-down and bimolecular fluorescence complementation (BiFC) assays. Gene expression analysis showed that AtMYB44 negatively regulated the expression of ABA-responsive gene RAB18, in contrast to the opposite role reported for RCAR1/PYL9. Competitive GST pull-down assay and analysis of phosphatase activity demonstrated that AtMYB44 and ABI1 competed for binding to RCAR1/PYL9 and thereby reduced the inhibitory effect of RCAR1/PYL9 on ABI1 phosphatase activity in the presence of ABA in vitro. Furthermore, transient activation assay in protoplasts revealed AtMYB44 probably also decreased RCAR1/PYL9-mediated inhibition of ABI1 activity in vivo."

According to the news editors, the research concluded: "Taken together, our work provides a reasonable molecular mechanism of AtMYB44 in ABA signaling."

For more information on this research see: Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44. International Journal of Molecular Sciences, 2014;15(5):8473-8490. International Journal of Molecular Sciences can be contacted at: Mdpi Ag, Postfach, Ch-4005 Basel, Switzerland (see also Proteins).

The news editors report that additional information may be obtained by contacting D.K. Li, Chinese Academy Agr Sci, Biotechnol Res Inst, Key Lab Crop Biotechnol, Minist Agr, Beijing 100081, People's Republic of China. Additional authors for this research include Y. Li, L. Zhang, X.Y. Wang, Z. Zhao, Z.W. Tao, J.M. Wang, J. Wang, M. Lin, X.F. Li and Y. Yang.

Keywords for this news article include: Asia, Beijing, Proteins, Transcription Factors, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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