News Column

Researchers' Work from Cornell University Focuses on Analytical Toxicology (A Rapid Screen for Four Corticosteroids in Equine Synovial Fluid)

July 8, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Life Science Research. According to news reporting from Ithaca, New York, by NewsRx journalists, research stated, "Most antidoping method development in the equine industry has been for plasma and urine, though there has been recent interest in the analysis of synovial fluid for evidence of doping by intra-articular corticosteroid injection. Published methods for corticosteroid analysis in synovial fluid are primarily singleplex methods, do not screen for all corticosteroids of interest and are not adequately sensitive."

The news correspondents obtained a quote from the research from Cornell University, "The purpose of this study is to develop a rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) screening method for the detection of four of the most common intra-articularly administered corticosteroids-betamethasone, methylprednisolone, methylprednisolone acetate and triamcinolone acetonide. Sample preparation consisted of protein precipitation followed by a basified liquid-liquid extraction. LC-MS-MS experiments consisted of a six-min isocratic separation using a Phenomenex Polar-RP stationary phase and a mobile phase consisting of 35% acetonitrile, 5 mM ammonium acetate and 0.1% formic acid in nanopure water. The detection system used was a triple quadrupole mass analyzer with thermospray ionization, and compounds were identified using selective reaction monitoring. The method was validated to the ISO/IEC 17025 standard, and real synovial fluid samples were analyzed to demonstrate the application of the method in an antidoping context. The method was highly selective for the four corticosteroids with limits of detection of 1-3 ng/mL. The extraction efficiency was 50-101%, and the matrix effects were 14-31%."

According to the news reporters, the research concluded: "These results indicate that the method is a rapid and sensitive screen for the four corticosteroids in equine synovial fluid, fit for purpose for equine antidoping assays."

For more information on this research see: A Rapid Screen for Four Corticosteroids in Equine Synovial Fluid. Journal of Analytical Toxicology, 2014;38(5):272-279. Journal of Analytical Toxicology can be contacted at: Oxford Univ Press Inc, Journals Dept, 2001 Evans Rd, Cary, NC 27513, USA. (Oxford University Press -; Journal of Analytical Toxicology -

Our news journalists report that additional information may be obtained by contacting K. Agrawal, Cornell University, Coll Vet Med, Dept. of Populat Med & Diagnost Sci, Ithaca, NY 14853, United States. Additional authors for this research include J.G. Ebel and K. Bischoff (see also Life Science Research).

Keywords for this news article include: Ithaca, New York, United States, Life Science Research, North and Central America

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Source: Life Science Weekly

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