News Column

Researchers Submit Patent Application, "Biological Detecting Chip", for Approval

July 8, 2014

By a News Reporter-Staff News Editor at Life Science Weekly -- From Washington, D.C., NewsRx journalists report that a patent application by the inventors SU, YU CHENG (Dayuan Township, TW); LEE, CHIA-YING (Taipei City, TW); CHANG, CHIAO-TUNG (Douliu City, TW); CHEN, CHENG HAN (New Taipei City, TW), filed on January 18, 2013, was made available online on June 26, 2014 (see also Ardic Instruments Co.).

The patent's assignee is Ardic Instruments Co.

News editors obtained the following quote from the background information supplied by the inventors: "The present invention relates to a biological detecting chip, particularly to a biological detecting chip for detecting optical fiber with nanoparticles.

"A lab-on-a-chip is an effective device that disposes a plurality of fluidic channels thereon and is able to integrate more than one experiment on such a single chip or to perform a high-throughput detection of biological sample. Interactions between biomolecules such as proteins, DNAs, or RNAs could be effectively analyzed inside small fluidic channels of the chip.

"One existing new technology is named Fiber Optical Particle Plasmon Resonance (FOPPR). An optical-fiber is utilized in the apparatus for detecting biological organisms in nano-scale. When an optical signal passes through the optical fiber, the light will be absorbed via Surface Plasma Resonance (SPR) effect induced by gold nanoparticles which resulted from the interaction of biological molecules, so as to detect various biological characteristics of proteins or bio-organisms and to be the biologically experimental base of immunoassay. Practically, FOPPR can be applied to do quantitative or kinetic analyses of proteins DNAs, RNAs or other small particles. Notably, it takes only one kind of antibody each time for FOPPR to achieve a highly sensitive quantitative analysis of proteins. FOPPR system utilizes the concept of Lab-on-a-chip and has an optical fiber disposed inside a fluidic channel to conduct experiments of interaction between biomolecules; to elaborate, FORRP has gold nanoparticles coated on the sensing area of the optical fiber and has biological ligands immobilized thereon. When the analytes contact with the biological ligands immobilized on the gold nanoparticles, the interaction between the biological samples and the biological ligands can be analyzed due to the signal variation (i.e., the wavelength shifting or variation of optical intensity), and a qualitative analysis or quantitative analysis of the biological samples can be carried out.

"Even though biosensors are promising to be used in various fields such as medical, pharmaceutical, environmental, defensive, bioprocessing, and food technological fields, the main obstacle for commercializing biosensors is bubbles stuck or accumulated in microfluidic channels. Surface roughness of the channel, the inappropriate microfluidic chamber design, and the turbulent flow that appears in the microfluidic channel all can lead to generation of bubbles. Moreover, in an optical detection system, undesired accumulation of bubbles in microfluidic channels can cause serious problems. The pressure and the flow rate in the microfluidic channel therefore change all the time and thus lead to system instability, which further devastates the ongoing analysis.

"In order to solve this problem, Changchun Liu et. al. ('A membrane-based, high-efficiency, microfluidic debubbler'. Lab Chip, 2011, Vol.11, p1688-1693) disclose a PTFE film with hydrophobic and porous membrane. The membrane is incorporated into the fluidic channel with altitude differences so that bubbles may be efficiently removed from the fluidic channel by means of the PTFE film and the pressure drop. In addition, Harald van Lintel et. al. ('High-Throughput Micro-Debubblers for Bubble Removal with Sub-Microliter Dead Volume', Micromachines, 2012, Vol.3 (2), p218-224) demonstrate a hydrophobic, permeable and water-resistant material. In this invention, bubbles are urged to pass through the hydrophobic material due to their greater buoyancy and then are removed from the fluidic channel. Yet, Jong Hwan Sung et. al. ('Prevention of air bubble formation in a microfluidic perfusion cell culture system using a microscale bubble trap', Biomedical Microdevices. 2009, Vol.11, p731-738) further demonstrate confining bubbles in a hole by a bubble trap; in this manner, bubbles would not be able to flow along with the fluid any longer, and the fluid is thus degassed.

"As mentioned, these well-known solutions are complicated and of low reproducibility. Intuitional observation, compact integration of chip, and de-bubbling, which can effectively enhance SPR phenomenon and still maintain sensitivity and accuracy of data, are substantially required in future developmental strategy."

As a supplement to the background information on this patent application, NewsRx correspondents also obtained the inventors' summary information for this patent application: "The primary object of the present invention is to resolve the problem of bubble accumulation in the fluidic channel of a biological detecting chip, so as to increase the SPR effect among the gold nanoparticles in the sensing area of the optical fiber and to accurately detect experimental data.

"To achieve the above purposes, a biological detecting chip is disclosed. The biological detecting chip comprises an optical fiber, at least one gas filter, an upper cap and a substrate. The optical fiber has at least one detecting area disposed on an outer surface. The upper cap has at least two guiding channels passed through the upper cap, at least one discharge channel with two ends connecting to an upper portion of distinct guiding channels, a inlet and an outlet, wherein the gas filter is attached to an upside of the discharge channel to separate the discharge channel from an outside of the upper cap. The substrate has a test area and a plurality of directing channels, wherein the directing channel connects to the inlet and the guiding channel, connects to the guiding channel and the test area, and connects to the test area and the outlet. The optical fiber is fixed between the upper cap and the substrate, with the detecting area disposed inside the test area and having an optical axis which crosses the directing channel by an angle.

"According to one embodiment of the biological detecting chip, wherein an upper surface of the upper cap has at least one receiving room disposed next to the discharge channel and selectively containing the gas filter.

"According to one embodiment of the biological detecting chip, wherein the guiding channel is vertically disposed.

"According to one embodiment of the biological detecting chip, wherein the directing channel is horizontally disposed.

"According to one embodiment of the biological detecting chip, wherein the number of the gas filter and the discharge channel are pluralities, and each of the directing channels connects to distinct guiding channels.

"According to one embodiment of the biological detecting chip, wherein the angle ranges from 1 to 90 degrees.

"According to one embodiment of the biological detecting chip, wherein the substrate has at least one wall to isolate and encircle the directing channel. The wall either protrudes or has a higher altitude than an upper surface of the substrate.

"According to one embodiment of the biological detecting chip, wherein the substrate has at least one wall to isolate and encircle the directing channel. An outside of the wall has a trough concaved and disposed next to the wall.

"According to one embodiment of the biological detecting chip, wherein the substrate has a plurality of fitting elements fastened to the upper cap or passed through the upper cap.

"The biological detecting chip according to the present invention may effectively control the generation of bubbles inside the channel of the chip. Therefore, the plasma effect of the gold nanoparticles on the optical fiber is increased, and the biochip is improved in its sensing accuracy of experimental signals. Thus the commercialization of the present invention is predictable.

"To further understand the techniques, means and effects of the instant disclosure applied for achieving the prescribed objectives, the following detailed descriptions and appended drawings are hereby referred, such that, through which, the purposes, features and aspects of the instant disclosure can be thoroughly and concretely appreciated. However, the appended drawings are provided solely for reference and illustration, without any intention to limit the instant disclosure.


"FIG. 1 is an exploded-view diagram of the biological detecting chip of the present invention;

"FIG. 2A-2C are schematic diagrams of the biological detecting chip after being assembled;

"FIG. 3 is schematic diagrams of the working fluid flowing inside the biological detecting chip;

"FIG. 4 is schematic diagrams showing the disposition of the wall and the trough of the biological detecting chip."

For additional information on this patent application, see: SU, YU CHENG; LEE, CHIA-YING; CHANG, CHIAO-TUNG; CHEN, CHENG HAN. Biological Detecting Chip. Filed January 18, 2013 and posted June 26, 2014. Patent URL:

Keywords for this news article include: Nanoparticle, Nanotechnology, Ardic Instruments Co., Emerging Technologies.

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Source: Life Science Weekly

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