Researchers from Washington University Describe Findings in Organic Chemistry (Imaging mRNA expression levels in living cells with PNA·DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Life Science Research. According to news reporting from St. Louis, Missouri, by NewsRx journalists, research stated, "Optical imaging of gene expression through the use of fluorescent antisense probes targeted to the mRNA has been an area of great interest. The main obstacles to developing highly sensitive antisense fluorescent imaging agents have been the inefficient intracellular delivery of the probes and high background signal from unbound probes."
The news correspondents obtained a quote from the research from Washington University, "Binary antisense probes have shown great promise as mRNA imaging agents because a signal can only occur if both probes are bound simultaneously to the mRNA target site. Selecting an accessible binding site is made difficult by RNA folding and protein binding in vivo and the need to bind two probes. Even more problematic, has been a lack of methods for efficient cytoplasmic delivery of the probes that would be suitable for eventual applications in vivo in animals. Herein we report the imaging of iNOS mRNA expression in live mouse macrophage cells with PNA·DNA binary FRET probes delivered by a cationic shell crosslinked knedel-like nanoparticle (cSCK). We first demonstrate that FRET can be observed on in vitro transcribed mRNA with both the PNA probes and the PNA·DNA hybrid probes."
According to the news reporters, the research concluded: "We then demonstrate that the FRET signal can be observed in live cells when the hybrid probes are transfected with the cSCK, and that the strength of the FRET signal is sequence specific and depends on the mRNA expression level."
For more information on this research see: Imaging mRNA expression levels in living cells with PNA·DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles. Organic and Biomolecular Chemistry, 2013;11(19):3159-67. (Royal Society of Chemistry - www.rsc.org/; Organic and Biomolecular Chemistry - pubs.rsc.org/en/journals/journalissues/ob)
Our news journalists report that additional information may be obtained by contacting Z. Wang, Dept. of Chemistry, Washington University, St Louis, MO 63130, United States. Additional authors for this research include K. Zhang, Y. Shen, J. Smith, S. Bloch, S. Achilefu, K.L. Wooley and J.S Taylor (see also Life Science Research).
Keywords for this news article include: Missouri, St. Louis, United States, Life Science Research, North and Central America.
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