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Researchers from Sichuan Agricultural University Report Recent Findings in Microbiology and Biotechnology (Improving endoglucanase activity by adding...

July 9, 2014



Researchers from Sichuan Agricultural University Report Recent Findings in Microbiology and Biotechnology (Improving endoglucanase activity by adding the carbohydrate-binding module from Corticium rolfsii)

By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biotechnology. According to news reporting originating from Ya'an, People's Republic of China, by NewsRx correspondents, research stated, "The carbohydrate-binding module (CBM) is an important domain of most cellulases that plays a key role in the hydrolysis of cellulose. The neutral endoglucanase (EG1) gene was reconstructed."

Our news editors obtained a quote from the research from Sichuan Agricultural University, "A redesigned endoglucanase, named EG2, was constructed with a CBM containing a linker from Corticium rolfsii (GenBank Accession No. D49448). The redesigned EG genes were expressed in Escherichia coli, and their characteristics are discussed. Results showed that the degradation of cellulose by EG2 was about double that by EG1. The specific activities of EG1 and EG2 were tested under optimal conditions, and EG2 had higher activity (169.1 2.74 U/mg) toward CMC-Na than did EG1 (84.0 1.98) in the process of cellulose degradation. The optimal pH and temperature, pH stability, and heat stability of EG1 and EG2 were similar. Results indicated that the CBM plays an essential role in the hydrolysis of cellulose."

According to the news editors, the research concluded: "We can improve EG's catalytic power by adding the CBM from Corticium rolfsii."

For more information on this research see: Improving endoglucanase activity by adding the carbohydrate-binding module from Corticium rolfsii. Journal of Microbiology and Biotechnology, 2014;24(4):440-6 (see also Biotechnology).

The news editors report that additional information may be obtained by contacting Z. Tang, College of Life and physical Sciences, Sichuan Agricultural University, Yaan 625014, People's Republic of China. Additional authors for this research include H. Chen, L. Chen, S. Liu, X. Han and Q. Wu.

Keywords for this news article include: Asia, Biotechnology, Ya'an, People's Republic of China.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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