Researchers from Korea Advanced Institute of Science and Technology (KAIST) Discuss Findings in Proteomics (Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas ...)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Proteomics is now available. According to news reporting out of Taejon, South Korea, by NewsRx editors, research stated, "Microalgae-derived biomass has been considered as the most promising candidate for next generation biofuel due to its sustainability and biodegradability. In this study, microalgal strain Chlamydmonas reinhardtii was randomly mutagenized by using a chemical mutagen, ethyl methane sulfonate (EMS) to create mutants showing enhanced lipid production."
Our news journalists obtained a quote from the research from the Korea Advanced Institute of Science and Technology (KAIST), "We identified three random mutants that displayed high lipid production in the screening using Nile red staining. Among those, mutant #128 was selected as candidate for further studies. Our flow cytometry and confocal microscopy analysis revealed that mutant #128 contains larger and more abundant lipid bodies than that of wild-type. Moreover, mutant #128 showed 1.4-fold increased fatty acid methyl ester (FAME) content compared to wild-type under nitrogen depleted condition. In addition, mutant #128 grew faster and accumulated more biomass, resulting in high lipid production. 2D gel electrophoresis and MALDI-TOF analysis used for gene targeting revealed that beta-subunit of mitochondrial ATP Synthase and two-component response regulator PilR may be involved in enhanced characteristics of mutant #128."
According to the news editors, the research concluded: "These results show the possibilities of EMS mediated random mutagenesis in generation of mutants to produce high amount of lipid as well as further study for molecular mechanism of mutants."
For more information on this research see: Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii. Korean Journal of Chemical Engineering, 2014;31(6):1036-1042. Korean Journal of Chemical Engineering can be contacted at: Korean Institute Chemical Engineers, F.5, 119, Anam-Ro, Seongbuk-Gu, Seoul 136-075, South Korea. (Springer - www.springer.com; Korean Journal of Chemical Engineering - www.springerlink.com/content/0256-1115/)
Our news journalists report that additional information may be obtained by contacting B. Lee, Korea Adv Inst Sci & Technol, Taejon 305701, South Korea. Additional authors for this research include G.G. Choi, Y.E. Choi, M. Sung, M.S. Park and J.W. Yang (see also Proteomics).
Keywords for this news article include: Asia, Taejon, Alkanes, Methane, Proteomics, South Korea
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