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Researchers at University of Toledo Report Findings in Stem Cells (Mechanical properties of human amniotic fluid stem cells using nanoindentation)

July 7, 2014

By a News Reporter-Staff News Editor at Stem Cell Week -- A new study on Stem Cell Research is now available. According to news reporting originating from Toledo, Ohio, by NewsRx correspondents, research stated, "The aim of this study was to obtain nanomechanical properties of living cells focusing on human amniotic fluid stem (hAFS) cell using nanoindentation techniques. We modified the conventional method of atomic force microscopy (AFM) in aqueous environment for cell imaging and indentation to avoid inherent difficulties."

Our news editors obtained a quote from the research from the University of Toledo, "Moreover, we determined the elastic modulus of murine osteoblast (OB6) cells and hAFS cells at the nucleus and cytoskeleton using force-displacement curves and Hertz theory. Since OB6 cell line has been widely used, it was selected to validate and compare the obtained results with the previous research studies. As a result, we were able to capture high resolution images through utilization of the tapping mode without adding protein or using fixation methods. The maximum depth of indentation was kept below 15% of the cell thickness to minimize the effect of substrate hardness. Nanostructural details on the surface of cells were visualized by AFM and fluorescence microscopy. The cytoskeletal fibers presented remarkable increase in elastic modulus as compared with the nucleus. Furthermore, our results showed that the elastic modulus of hAFS cell edge (31.6 kPa) was lower than that of OB6 cell edge (42.2 kPa). In addition, the elastic modulus of nucleus was 13.9 kPa for hAFS cell and 26.9 kPa for OB6 cells."

According to the news editors, the research concluded: "Differences in cell elastic modulus possibly resulted from the type and number of actin cytoskeleton organization in these two cell types."

For more information on this research see: Mechanical properties of human amniotic fluid stem cells using nanoindentation. Journal of Biomechanics, 2013;46(9):1524-30. (Elsevier -; Journal of Biomechanics -

The news editors report that additional information may be obtained by contacting A. Aryaei, Dept. of Mechanical Engineering, University of Toledo, 1650 NWestwood Avenue, Toledo, OH 43606-3390, United States (see also Stem Cell Research).

Keywords for this news article include: Ohio, Toledo, United States, Nanotechnology, Nanoindentation, Stem Cell Research, Emerging Technologies, North and Central America.

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Source: Stem Cell Week

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