Research Results from Chongqing Medical University Update Knowledge of DNA Research (DNA-AuNPs based signal amplification for highly sensitive detection of DNA methylation, methyltransferase activity and inhibitor screening)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on DNA Research is now available. According to news reporting originating in Chongqing, People's Republic of China, by NewsRx journalists, research stated, "A sensitive and selective electrochemical method was developed for the detection of DNA methylation, determination of DNA methyltransferase (MTase) activity and screening of MTase inhibitor. Methylene blue (MB) was employed as electrochemical indicator and DNA-modified gold nanoparticles (AuNPs) were used as signal amplification unit because the DNA strands in this composite have strong adsorption ability for MB."
The news reporters obtained a quote from the research from Chongqing Medical University, "First, the thiolated single-stranded DNA S1 was self-assembled on gold electrode, hybridization between the lower portion of DNA S1 and its complementary DNA S2 formed an identical double-stranded tetranucleotide target sequence for both DNA adenine methylation (Dam) MTase and methylation-resistant endonuclease Mbo I, then the upper portion of DNA S1 was hybridized with its complementary DNA S3 modified on AuNPs to bring the DNA S3-AuNPs amplification units onto the electrode. The DNA S1/S2/S3-AuNPs bioconjugate has lots of DNA strands, and they can adsorb abundant MB. Mbo I endounuclease could not cleave the identical target sequence after it was methylated by Dam MTase. On the contrary, the sequence without methylation could be cleaved, which would decrease the amount of adsorbed MB. The presence of redox-active MB was detected electrochemically by differential pulse voltammetry (DPV). Thus, the activity of Dam MTase and methylation status were sensitively converted to the DNA S3-AuNPs amplified DPV signals. The DPV signal demonstrated a linear relationship with logarithm of Dam concentration ranging from 0.075 to 30U/mL, achieving a detection limit of 0.02U/mL (S/N=3)."
According to the news reporters, the research concluded: "Also, screening of Dam MTase inhibitor 5-fluorouracil was successfully investigated using this fabricated sensor."
For more information on this research see: DNA-AuNPs based signal amplification for highly sensitive detection of DNA methylation, methyltransferase activity and inhibitor screening. Biosensors & Bioelectronics, 2014;58():40-7. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news correspondents report that additional information may be obtained by contacting X. Jing, Key Laboratory of Laboratory Medical Diagnostics of Education, Dept. of Laboratory Medicine, Chongqing Medical University, No 1 Yi Xue Yuan Road, Chongqing 400016, People's Republic of China. Additional authors for this research include X. Cao, L. Wang, T. Lan, Y. Li and G. Xie (see also DNA Research).
Keywords for this news article include: Asia, Chongqing, Chemistry, DNA Research, Electrochemical, People's Republic of China.
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