Reports from Texas A&M University Add New Data to Findings in Surface-Active Agents (Cold-microwave enhanced enzyme-linked immunosorbent assays-A path to high-throughput clinical diagnostics)
By a News Reporter-Staff News Editor at Life Science Weekly -- A new study on Surface-Active Agents is now available. According to news reporting originating in College Station, Texas, by NewsRx journalists, research stated, "The enzyme-linked immunosorbent assay (ELISA) constitutes an important clinical diagnostic approach. However, the prolonged incubation times involved lead to turnaround times of typically >= day, potentially delaying a definitive diagnosis or an adequate treatment plan for individual patients."
The news reporters obtained a quote from the research from Texas A&M University, "Here cold-microwave technology (CMT) was employed to significantly reduce the times required for diagnostic ELISAs. The new approach was validated and compared to a conventional ELISA setup measuring canine calprotectin (cCP). Canine serum and fecal specimens were used for the analytical validation of cCP ELISA by conventional and CMT ELISA. Cross-validation of both ELISA methods consisted of the determination of analytic sensitivity, linearity, accuracy, precision, and reproducibility. The long-term stability of antibodycoated ELISA plates was also evaluated up to 33 days. The ELISA approaches were comparable to each other. The observed-to-expected ratios for linearity and accuracy were 100.2 +/- 11.8 and 98.1 +/- 10.8% (mean +/- standard deviation), respectively. Precision and reproducibility were
According to the news reporters, the research concluded: "While both ELISA approaches were analytically sensitive, linear, accurate, precise, and reproducible with measurements of cCP concentrations, CMT ELISA offered a reduction in incubation times by 90-95%, facilitating a very fast turnaround time and suggesting CMT ELISA for improved human and veterinary clinical diagnostics."
For more information on this research see: Cold-microwave enhanced enzyme-linked immunosorbent assays-A path to high-throughput clinical diagnostics. Analytical Biochemistry, 2014;457():65-73. Analytical Biochemistry can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Analytical Biochemistry - www.elsevier.com/wps/product/cws_home/622781)
Our news correspondents report that additional information may be obtained by contacting N. Grutzner, Texas A&M University, Dept. of Biochem & Biophys, College Stn, TX 77843, United States. Additional authors for this research include R.M. Heilmann, J.S. Suchodolski, J.M. Steiner and A. Holzenburg (see also Surface-Active Agents).
Keywords for this news article include: Texas, United States, College Station, Immunologic Tests, Enzymes and Coenzymes, Surface-Active Agents, Immunologic Techniques, Immunoenzyme Techniques, Immunosorbent Techniques, Investigative Techniques, North and Central America, Enzyme-Linked Immunosorbent Assay
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