Recent Findings from University of Pennsylvania Provide New Insights into Serum Globulins (Chemically grafted fibronectin for use in QCM-D cell studies)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Proteins. According to news reporting originating from Philadelphia, Pennsylvania, by NewsRx correspondents, research stated, "Traditionally, fibronectin has been used as a physisorbed surface coating (physFN) in cell culture experiments due to its critical role in cell adhesion. However, because the resulting layer is thick, unstable, and of unpredictable uniformity, this method of fibronectin deposition is unsuitable for some types of research, including quartz crystal microbalance (QCM) experiments involving cells."
Our news editors obtained a quote from the research from the University of Pennsylvania, "Here, we present a new method for chemical immobilization of fibronectin onto silicon oxide surfaces, including QCM crystals pre-coated with silicon oxide. We characterize these chemically coated fibronectin surfaces (chemFN) as well as physFN ones using spectroscopic ellipsometry (SE), Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. A cell culture model demonstrates that cells on chemFN and physFN surfaces exhibit similar viability, structure, adhesion and metabolism. Finally, we perform QCM experiments using cells on both surfaces which demonstrate the superior suitability of chemFN coatings for QCM research, and provide real-time QCM-D data from cells subjected to an actin depolymerizing agent. Overall, our method of chemical immobilization of fibronectin yields great potential for furthering cellular experiments in which thin, stable and uniform coatings are desirable."
According to the news editors, the research concluded: "As QCM research with cells has been rather limited in success thus far, we anticipate that this new technique will particularly benefit this experimental system by availing it to the much broader field of cell mechanics."
For more information on this research see: Chemically grafted fibronectin for use in QCM-D cell studies. Biosensors & Bioelectronics, 2014;58():249-57. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
The news editors report that additional information may be obtained by contacting J. Kandel, Dept. of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, United States. Additional authors for this research include H.S. Lee, P. Sobolewski, N. Tomczyk, R.J. Composto and D.M Eckmann (see also Proteins).
Keywords for this news article include: Philadelphia, Pennsylvania, Fibronectins, United States, Serum Globulins, Membrane Glycoproteins, North and Central America, Extracellular Matrix Proteins.
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