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New Esterases Data Have Been Reported by Investigators at Chongqing University of Technology (Electrochemiluminescence recovery-based aptasensor for...

July 8, 2014



New Esterases Data Have Been Reported by Investigators at Chongqing University of Technology (Electrochemiluminescence recovery-based aptasensor for sensitive Ochratoxin A detection via exonuclease-catalyzed target recycling amplification)

By a News Reporter-Staff News Editor at Life Science Weekly -- Data detailed on Enzymes and Coenzymes have been presented. According to news reporting from Chongqing, People's Republic of China, by NewsRx journalists, research stated, "Based on the recovery of the quantum dot (QD) electrochemiluminescence (ECL) and exonuclease-catalyzed target recycling amplification, the development of a highly sensitive aptasensor for Ochratoxin A (OTA) detection is described. The duplex DNA probes containing the biotin-modified aptamer are immobilized on a CdTe QD composite film-coated electrode."

The news correspondents obtained a quote from the research from the Chongqing University of Technology, "The presence of the OTA target leads to effective removal of the biotin-aptamers from the electrode surface via exonuclease-catalyzed recycling and reuse of OTA, which prevents the attachment of streptavidin-alkaline phosphatase (STV-ALP) through biotin-STV interaction. The electron transfer (ET) from the excited state CdTe QD ([CdTe]*) to the electro-oxidized species of the enzymatic product of ALP during the potential scan is thus inhibited and the QD ECL emission is restored for quantitative OTA detection. Due to the exonuclease-catalyzed target recycling amplification, the inhibition effect of ET is significantly enhanced to achieve sensitive detection of OTA down to 0.64 pg mL(-1). The proposed method is selective for OTA and can be used to monitor OTA in real red wine samples."

According to the news reporters, the research concluded: "Our developed ECL recovery-based aptasensor thus offers great potential for the development of new ECL sensing platforms for various target analytes."

For more information on this research see: Electrochemiluminescence recovery-based aptasensor for sensitive Ochratoxin A detection via exonuclease-catalyzed target recycling amplification. Talanta, 2014;125():45-50. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)

Our news journalists report that additional information may be obtained by contacting M.L. Yang, Chongqing Univ Technol, Sch Chem & Chem Engn, Chongqing 400054, People's Republic of China. Additional authors for this research include B.Y. Jiang, J.Q. Xie, Y. Xiang, R. Yuan and Y.Q. Chai (see also Enzymes and Coenzymes).

Keywords for this news article include: Asia, Biotin, Chongqing, Esterases, Mycotoxins, Ochratoxins, Exonucleases, Micronutrient, Diet and Nutrition, Enzymes and Coenzymes, People's Republic of China

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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