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New DNA Research Findings from University of Texas Discussed (3D Structural Integrity and Interactions of Single-Stranded Protein-Binding DNA in a...

July 8, 2014



New DNA Research Findings from University of Texas Discussed (3D Structural Integrity and Interactions of Single-Stranded Protein-Binding DNA in a Functionalized Nanopore)

By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting from Arlington, Texas, by NewsRx journalists, research stated, "Biomarker-binding nucleotide sequences, like aptamers, have gained recent attention in cancer cell isolation and detection works. Self-assembly and 3D conformation of aptamers enable them to selectively capture and bind diseased cells and related biomarkers."

The news correspondents obtained a quote from the research from the University of Texas, "One mode of utilizing such an extraordinary selective property of the aptamers is by grafting these in nanopores. Coating the inside walls of the nanopore with biomarker specific ligands, like DNA, changes the statistics of the dynamic translocation events. When the target protein passes through the nanopore, it interacts with ligand coated inside the nanopore, and the process alters the overall potential energy profile which is essentially specific to the protein detected. The fundamental goal in this process is to ensure that these detection motifs hold their structure and functionality under applied electric field and experimental conditions. We report here all-atom molecular dynamics simulations of the effects of external electric field on the 3D conformation of such DNA structures. The simulations demonstrate how the grafted moieties affect the translocation time, velocity, and detection frequency of the target molecule. We also investigated a novel case of protein translocation, where DNA is prebound to the protein."

According to the news reporters, the research concluded: "As model, a thrombin-specific G-quartet and thrombin pair was used for this study."

For more information on this research see: 3D Structural Integrity and Interactions of Single-Stranded Protein-Binding DNA in a Functionalized Nanopore. Journal of Physical Chemistry B, 2014;118(22):5799-5806. Journal of Physical Chemistry B can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Physical Chemistry B - www.pubs.acs.org/journal/jpcbfk)

Our news journalists report that additional information may be obtained by contacting M.A.I. Mahmood, Univ Texas Arlington, Univ Texas SW Med Center Dallas, Arlington, TX 76019, United States. Additional authors for this research include W. Ali, A. Adnan and S.M. Iqbal (see also DNA Research).

Keywords for this news article include: Texas, Arlington, DNA Research, United States, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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