News Column

Investigators from Aalborg University Target Geobacter (Physiology of Geobacter metallireducens under excess and limitation of electron donors. Part...

July 8, 2014



Investigators from Aalborg University Target Geobacter (Physiology of Geobacter metallireducens under excess and limitation of electron donors. Part II. Mimicking environmental conditions during cultivation in retentostats)

By a News Reporter-Staff News Editor at Life Science Weekly -- Investigators publish new report on Proteobacteria. According to news originating from Aalborg, Denmark, by NewsRx correspondents, research stated, "The strict anaerobe Geobacter metallireducens was cultivated in retentostats under acetate and acetate plus benzoate limitation in the presence of Fe(III) citrate in order to investigate its physiology under close to natural conditions. Growth rates below 0.003 h(-1) were achieved in the course of cultivation."

Our news journalists obtained a quote from the research from Aalborg University, "A nano-liquid chromatography-tandem mass spectrometry-based proteomic approach (nano-LC-MS/MS) with subsequent label-free quantification was performed on proteins extracted from cells sampled at different time points during retentostat cultivation. Proteins detected at low (0.002 h(-1)) and high (0.06 h(-1)) growth rates were compared between corresponding growth conditions (acetate or acetate plus benzoate). Carbon limitation significantly increased the abundances of several catabolic proteins involved in the degradation of substrates not present in the medium (ethanol, butyrate, fatty acids, and aromatic compounds). Growth rate-specific physiology was reflected in the changed abundances of energy-, chemotaxis-, oxidative stress-, and transport-related proteins."

According to the news editors, the research concluded: "Mimicking natural conditions by extremely slow bacterial growth allowed to show how G. metallireducens optimized its physiology in order to survive in its natural habitats, since it was prepared to consume several carbon sources simultaneously and to withstand various environmental stresses."

For more information on this research see: Physiology of Geobacter metallireducens under excess and limitation of electron donors. Part II. Mimicking environmental conditions during cultivation in retentostats. Systematic and Applied Microbiology, 2014;37(4):287-295. Systematic and Applied Microbiology can be contacted at: Elsevier Gmbh, Urban & Fischer Verlag, Office Jena, P O Box 100537, 07705 Jena, Germany. (Elsevier - www.elsevier.com; Systematic and Applied Microbiology - www.elsevier.com/wps/product/cws_home/701801)

The news correspondents report that additional information may be obtained from S. Marozava, Aalborg Univ, Dept. of Biotechnol Chem & Environm Engn, DK-9000 Aalborg, Denmark. Additional authors for this research include W.F.M. Roling, J. Seifert, R. Kuffner, M. von Bergen and R.U. Meckenstock (see also Proteobacteria).

Keywords for this news article include: Europe, Aalborg, Denmark, Geobacter, Deltaproteobacteria, Gram-Negative Bacteria, Gram-Negative Anaerobic Bacteria

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


For more stories covering the world of technology, please see HispanicBusiness' Tech Channel



Source: Life Science Weekly


Story Tools






HispanicBusiness.com Facebook Linkedin Twitter RSS Feed Email Alerts & Newsletters