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Findings on Connective Tissue Cells Detailed by Investigators at University of Wroclaw (Efficient production and purification of extracellular domain...

July 7, 2014



Findings on Connective Tissue Cells Detailed by Investigators at University of Wroclaw (Efficient production and purification of extracellular domain of human FGFR-Fc fusion proteins from Chinese hamster ovary cells)

By a News Reporter-Staff News Editor at Proteomics Weekly -- Current study results on Connective Tissue Cells have been published. According to news reporting originating in Wroclaw, Poland, by NewsRx journalists, research stated, "The family of fibroblast growth factor receptors (FGFRs) plays an important role in cell growth, survival, differentiation and angiogenesis. The three immunoglobulin-like extracellular domains of FGFR (D1, D2, and D3) are critical for ligand binding and specificity towards fibroblast growth factor and heparan sulfate."

The news reporters obtained a quote from the research from the University of Wroclaw, "Fibroblast growth factor receptors are overexpressed in a wide variety of tumors, such as breast, bladder, and prostate cancer, and therefore they are attractive targets for different types of anticancer therapies. In this study, we have cloned, expressed in CHO cells and purified Fc-fused extracellular domains of different types of FGFRs (ECD_FGFR1a-Fc, ECD_FGFR1b-Fc, ECD_FGFR2a-Fc, ECD_FGFR2b-Fc, ECD_FGFR3a-Fc, ECD_FGFR3b-Fc, ECD_FGFR4a-Fc, ECD_FGFR4b-Fc), which could be used as molecular targets for the selection of specific antibodies. The fusion proteins were analyzed using gel electrophoresis, Western blotting and mass spectrometry. To facilitate their full characterization, the fusion proteins were deglycosylated using PNGase F enzyme."

According to the news reporters, the research concluded: "With an optimized transient transfection protocol and purification procedure we were able to express the proteins at a high level and purify them to homogeneity."

For more information on this research see: Efficient production and purification of extracellular domain of human FGFR-Fc fusion proteins from Chinese hamster ovary cells. Protein Expression and Purification, 2014;99():50-57. Protein Expression and Purification can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Protein Expression and Purification - www.elsevier.com/wps/product/cws_home/622935)

Our news correspondents report that additional information may be obtained by contacting A. Sokolowska-Wedzina, University of Wroclaw, Fac Biotechnol, Dept. of Prot Engn, PL-50383 Wroclaw, Poland. Additional authors for this research include A. Borek, J. Chudzian, P. Jakimowicz, M. Zakrzewska and J. Otlewski (see also Connective Tissue Cells).

Keywords for this news article include: Poland, Europe, Wroclaw, Fibroblasts, Connective Tissue Cells

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Proteomics Weekly


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