Findings from Shandong University Has Provided New Data on General Chemistry (Quantitative single-molecule detection of protein based on DNA tetrahedron fluorescent nanolabels)
By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on General Chemistry are presented in a new report. According to news reporting from Jinan, People's Republic of China, by NewsRx journalists, research stated, "A highly sensitive method for single-molecule quantitative detection of human IgG is presented by the employment of a new fluorescent nanolabel. In this method, fluorescent nanolabels were assembled by inserting SYBR Green I into DNA tetrahedron nanostructure."
The news correspondents obtained a quote from the research from Shandong University, "The bio-nanolabels were attached to the streptavidin-antihuman antibody by a specific reaction between biotin and streptavidin. The antibody was combined with the target antigen, human IgG, which was immobilized on the silanized glass subtrate surface. Finally, epi-fluorescence microscopy (EFM) coupled with an electron multiplying charge-coupled device was employed for fluorescence imaging. The fluorescent spots corresponding to single protein molecule on images were counted and further used for the quantitative detection. It was found that the new nanolabel shows good photostability, biocompatiblity and exhibits no blinking compared to traditional labels like fluorescence dyes and quantum dot (QDs). In addition, the number of fluorescence spots on the images has a linear relationship with the concentration of human IgG in the range of 3.0 x 10(-14) to 1.0 x 10(-12) mol L-1."
According to the news reporters, the research concluded: "What is more, this method showed an excellent specificity and a low matrix effect."
For more information on this research see: Quantitative single-molecule detection of protein based on DNA tetrahedron fluorescent nanolabels. Talanta, 2014;125():393-399. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
Our news journalists report that additional information may be obtained by contacting Y.S. Ding, Shandong University, Sch Pharm, Jinan 250012, People's Republic of China. Additional authors for this research include X.T. Liu, J. Zhu, L. Wang and W. Jiang (see also General Chemistry).
Keywords for this news article include: Asia, Jinan, General Chemistry, People's Republic of China
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