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Findings from National Institute for Occupational Safety and Health Has Provided New Data on Stem Cells (Induction of stem-like cells with malignant...

July 8, 2014



Findings from National Institute for Occupational Safety and Health Has Provided New Data on Stem Cells (Induction of stem-like cells with malignant properties by chronic exposure of human lung epithelial cells to single-walled carbon nanotubes)

By a News Reporter-Staff News Editor at Cancer Weekly -- A new study on Stem Cell Research is now available. According to news reporting originating from Morgantown, West Virginia, by NewsRx correspondents, research stated, "Carbon nanotubes (CNT) hold great promise to create new and better products for commercial and biomedical applications, but their long-term adverse health effects are a major concern. The objective of this study was to address human lung cancer risks associated with chronic pulmonary exposure to single-walled (SW) CNT through the fundamental understanding of cellular and molecular processes leading to carcinogenesis."

Our news editors obtained a quote from the research from National Institute for Occupational Safety and Health, "We hypothesized that the acquisition of cancer stem cells (CSC), a subpopulation that drive tumor initiation and progression, may contribute to CNT carcinogenesis. Non-tumorigenic human lung epithelial cells were chronically exposed to well-dispersed SWCNT for a period of 6 months at the physiologically relevant concentration of 0.02 mu g/cm(2) surface area dose. Chronic SWCNT-exposed cells were evaluated for the presence of CSC-like cells under CSC-selective conditions of tumor spheres and side population (SP). CSC-like cells were isolated using fluorescence-activated cell sorting and were assessed for aggressive behaviors, including acquired apoptosis resistance and increased cell migration and invasion in vitro, and tumor-initiating capability in vivo. Non-small cell lung cancer cells served as a positive control. We demonstrated for the first time the existence of CSC-like cells in all clones of chronic SWCNT-exposed lung epithelial cells. These CSC-like cells, in contrary to their non-CSC counterpart, possessed all biological features of lung CSC that are central to irreversible malignant transformation, self-renewal, aggressive cancer behaviors, and in vivo tumorigenesis. These cells also displayed aberrant stem cell markers, notably Nanog, SOX-2, SOX-17 and E-cadherin. Restored expression of tumor suppressor p53 abrogated CSC properties of CSC-like cells. Furthermore, we identified specific stem cell surface markers CD24(low) and CD133(high) that are associated with SWCNT-induced CSC formation and tumorigenesis. Our findings provide new and compelling evidence for the acquisition of CSC-like cells induced by chronic SWCNT exposure, which are likely to be a major driving force for SWCNT tumorigenesis. Thus, our study supports prudent adoption of prevention strategies and implementation of exposure control for SWCNT."

According to the news editors, the research concluded: "We also suggest that the detection of CSC and associated surface markers may provide an effective screening tool for prediction of the carcinogenic potential of SWCNT and related nanoparticles."

For more information on this research see: Induction of stem-like cells with malignant properties by chronic exposure of human lung epithelial cells to single-walled carbon nanotubes. Particle and Fibre Toxicology, 2014;11():2-18. Particle and Fibre Toxicology can be contacted at: Biomed Central Ltd, 236 Grays Inn Rd, Floor 6, London WC1X 8HL, England. (BioMed Central - www.biomedcentral.com/; Particle and Fibre Toxicology - www.particleandfibretoxicology.com)

The news editors report that additional information may be obtained by contacting S. Luanpitpong, NIOSH, Pathol & Physiol Res Branch, Morgantown, WV 26505, United States. Additional authors for this research include L.Y. Wang, V. Castranova and Y. Rojanasakul (see also Stem Cell Research).

Keywords for this news article include: Cancer, Oncology, Morgantown, Fullerenes, West Virginia, United States, Carcinogenesis, Carbon Nanotubes, Epithelial Cells, Stem Cell Research, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Cancer Weekly


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