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Findings from G.H. Cui and Co-Authors Provides New Data about Life Science Research (Deep brain optical measurements of cell type-specific neural...

July 8, 2014



Findings from G.H. Cui and Co-Authors Provides New Data about Life Science Research (Deep brain optical measurements of cell type-specific neural activity in behaving mice)

By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Life Science Research are discussed in a new report. According to news reporting out of Lisbon, Portugal, by NewsRx editors, research stated, "Recent advances in genetically encoded fluorescent sensors enable the monitoring of cellular events from genetically defined groups of neurons in vivo. In this protocol, we describe how to use a time-correlated single-photon counting (TCSPC)-based fiber optics system to measure the intensity, emission spectra and lifetime of fluorescent biosensors expressed in deep brain structures in freely moving mice."

Our news journalists obtained a quote from the research, "When combined with Cre-dependent selective expression of genetically encoded Ca2+ indicators (GECIs), this system can be used to measure the average neural activity from a specific population of cells in mice performing complex behavioral tasks. As an example, we used viral expression of GCaMPs in striatal projection neurons (SPNs) and recorded the fluorescence changes associated with calcium spikes from mice performing a lever-pressing operant task. The whole procedure, consisting of virus injection, behavior training and optical recording, takes 3-4 weeks to complete. With minor adaptations, this protocol can also be applied to recording cellular events from other cell types in deep brain regions, such as dopaminergic neurons in the ventral tegmental area."

According to the news editors, the research concluded: "The simultaneously recorded fluorescence signals and behavior events can be used to explore the relationship between the neural activity of specific brain circuits and behavior."

For more information on this research see: Deep brain optical measurements of cell type-specific neural activity in behaving mice. Nature Protocols, 2014;9(6):1213-1228. Nature Protocols can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group - www.nature.com/; Nature Protocols - www.nature.com/nprot/)

Our news journalists report that additional information may be obtained by contacting G.H. Cui, Champalimaud Center, Lisbon, Portugal. Additional authors for this research include S.B. Jun, X. Jin, G.X. Luo, M.D. Pham, D.M. Lovinger, S.S. Vogel and R.M. Costa (see also Life Science Research).

Keywords for this news article include: Lisbon, Europe, Portugal, Life Science Research

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Life Science Weekly


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