Study Data from University of Putra Malaysia Provide New Insights into DNA Research (Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles)
By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting originating in Selangor, Malaysia, by NewsRx journalists, research stated, "A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution."
The news reporters obtained a quote from the research from the University of Putra Malaysia, "Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra."
According to the news reporters, the research concluded: "The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA."
For more information on this research see: Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles. Journal of Nanomaterials, 2014;():1-5. Journal of Nanomaterials can be contacted at: Hindawi Publishing Corporation, 410 Park Avenue, 15TH Floor, #287 Pmb, New York, NY 10022, USA. (Hindawi Publishing - www.hindawi.com; Journal of Nanomaterials - www.hindawi.com/journals/jnm/)
Our news correspondents report that additional information may be obtained by contacting S.A. Rahman, University of Putra Malaysia, Inst Adv Technol, Serdang 43400, Selangor, Malaysia. Additional authors for this research include R. Saadun, N.E. Azmi, N. Ariffin, J. Abdullah, N.A. Yusof, H. Sidek and R. Hajian (see also DNA Research).
Keywords for this news article include: Asia, Selangor, Malaysia, Viral DNA, DNA Research, Nanotechnology, Gold Nanoparticles, Emerging Technologies
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