Studies in the Area of Purification Technology Reported from Dublin City University (Preparation and characterization of negatively charged organic-inorganic hybrid ultrafiltration membranes for protein separation)
By a News Reporter-Staff News Editor at Life Science Weekly -- New research on Purification Technology is the subject of a report. According to news originating from Dublin, Ireland, by NewsRx correspondents, research stated, "Negatively charged organic-inorganic hybrid ultrafiltration membranes with adjustable charge density were fabricated from blends of water soluble poly vinyl alcohol and N-O-sulfonic acid benzyl chitosan (NSBC) in combination with tetraethyl orthosilicate (TEOS) silica precursor by the sol-gel method and precipitation in a mixture of methanol and water (80 wt%:20 wt%) solvent. The porous hybrid membrane morphologies, structures and surface properties were characterized comprehensively using scanning electron microscopy, Fourier transform infrared spectroscopy in the attenuated total reflection mode, as well as contact angle measurements."
Our news journalists obtained a quote from the research from Dublin City University, "The results confirmed that the fabricated membranes were porous, hydrophilic and mildly charged in nature. The water flux and flux recovery ratio (i.e. protein fouling resistance) of the membranes were highly dependent on the fraction of NSBC. The protein transmission as a function of pH and the fraction of NSBC was studied for two model proteins (bovine serum albumin; BSA and lysozyme; LYZ) and found to be controlled by size exclusion and the fraction of NSBC, and therefore the membrane charge density. The highest transmission of proteins at their isoelectric points was obtained for the membrane containing 40 wt% of NSBC."
According to the news editors, the research concluded: "The best separation of LYZ from BSA in the model mixture solution was obtained at pH =11 when membrane C-40 was used in ultrafiltration of protein solution."
For more information on this research see: Preparation and characterization of negatively charged organic-inorganic hybrid ultrafiltration membranes for protein separation. Separation and Purification Technology, 2014;130():112-123. Separation and Purification Technology can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Separation and Purification Technology - www.elsevier.com/wps/product/cws_home/600251)
The news correspondents report that additional information may be obtained from M. Kumar, Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland (see also Purification Technology).
Keywords for this news article include: Dublin, Europe, Ireland, Purification Technology
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