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Studies from Musashino University Yield New Information about Biomedicine and Biomedical Engineering (Improved separation of fluorogenic derivatized...

July 23, 2014



Studies from Musashino University Yield New Information about Biomedicine and Biomedical Engineering (Improved separation of fluorogenic derivatized intact proteins with high resolution and efficiency using a reversed-phase liquid ...)

By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Biotechnology. According to news reporting out of Tokyo, Japan, by NewsRx editors, research stated, "Although the efficient separation of intact protein mixtures is extremely difficult, reversed-phase chromatography is an important technique for performing quantitative, accurate and reproducible proteomics analyses. Here, we show that, despite the operating constraints of conventional high-performance liquid chromatography, such as column temperature, operating pressure and separation time, comprehensive separation of fluorogenic derivatized intact proteins could be achieved with high resolution and separation efficiency."

Our news journalists obtained a quote from the research from Musashino University, "First, amylin was chosen as a model peptide and used to estimate the separation efficiency with respect to column temperature and flow rate, as indicated by peak capacity. Then, an extract of human primary hepatocytes was used to model complex component mixtures and the separation conditions were optimized. The effects of mobile-phase pH, the separation time and the column length were also investigated. Consequently, more than 890 peaks could be separated efficiently in the extract, which is 1.5-fold greater than when using conventional conditions. Finally, it was demonstrated that both longer separation time and column length contributed greatly to the effective separation of the protein mixture."

According to the news editors, the research concluded: "These results are expected to provide insights into the separation of intact proteins."

For more information on this research see: Improved separation of fluorogenic derivatized intact proteins with high resolution and efficiency using a reversed-phase liquid chromatographic system. Biomedical Chromatography, 2014;28(6):862-867. Biomedical Chromatography can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Biomedical Chromatography - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-0801)

Our news journalists report that additional information may be obtained by contacting T. Ichibangase, Musashino Univ, Pharmaceut Sci Res Inst, Nishitokyo, Tokyo 2028585, Japan. Additional authors for this research include K. Nakata and K. Imai (see also Biotechnology).

Keywords for this news article include: Asia, Biotechnology, Tokyo, Japan

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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