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Studies from M. Bielecka and Colleagues Reveal New Findings on Plant Biotechnology (Targeted mutation of Delta 12 and Delta 15 desaturase genes in...

July 23, 2014



Studies from M. Bielecka and Colleagues Reveal New Findings on Plant Biotechnology (Targeted mutation of Delta 12 and Delta 15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil)

By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Biotechnology have been published. According to news originating from York, United Kingdom, by NewsRx correspondents, research stated, "We used expressed sequence tag library and whole genome sequence mining to identify a suite of putative desaturase genes representing the four main activities required for production of polyunsaturated fatty acids in hemp seed oil. Phylogenetic-based classification and developing seed transcriptome analysis informed selection for further analysis of one of seven Delta 12 desaturases and one of three Delta 15 desaturases that we designate CSFAD2A and CSFAD3A, respectively."

Our news journalists obtained a quote from the research, "Heterologous expression of corresponding cDNAs in Saccharomyces cerevisiae showed CSFAD2A to have Delta x+3 activity, while CSFAD3A activity was exclusively at the Delta 15 position. TILLING of an ethyl methane sulphonate mutagenized population identified multiple alleles including non-sense mutations in both genes and fatty acid composition of seed oil confirmed these to be the major Delta 12 and Delta 15 desaturases in developing hemp seed. Following four backcrosses and sibling crosses to achieve homozygosity, csfad2a-1 was grown in the field and found to produce a 70 molar per cent high oleic acid (18:1(Delta 9)) oil at yields similar to wild type. Cold-pressed high oleic oil produced fewer volatiles and had a sevenfold increase in shelf life compared to wild type. Two low abundance octadecadienoic acids, 18:2(Delta 6,9) and 18:2(Delta 9,15), were identified in the high oleic oil, and their presence suggests remaining endogenous desaturase activities utilize the increased levels of oleic acid as substrate. Consistent with this, CSFAD3A produces 18:2(Delta 9,15) from endogenous 18:1(Delta 9) when expressed in S. cerevisiae."

According to the news editors, the research concluded: "This work lays the foundation for the development of additional novel oil varieties in this multipurpose low input crop."

For more information on this research see: Targeted mutation of Delta 12 and Delta 15 desaturase genes in hemp produce major alterations in seed fatty acid composition including a high oleic hemp oil. Plant Biotechnology Journal, 2014;12(5):613-623. Plant Biotechnology Journal can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Plant Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1467-7652)

The news correspondents report that additional information may be obtained from M. Bielecka, York Sci Pk, Bioctr, Biorenewables Dev Center, York, N Yorkshire, United Kingdom. Additional authors for this research include F. Kaminski, I. Adams, H. Poulson, R. Sloan, Y. Li, T.R. Larson, T. Winzer and I.A. Graham (see also Biotechnology).

Keywords for this news article include: York, Biotechnology, Europe, Desaturase, United Kingdom, Enzymes and Coenzymes

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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