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Studies from J.O. Rojas-Reyes and Colleagues Yield New Information about Peroxidases [Purification and characterization of peroxidase from avocado...

July 24, 2014



Studies from J.O. Rojas-Reyes and Colleagues Yield New Information about Peroxidases [Purification and characterization of peroxidase from avocado (Persea americana Mill, cv. Hass)]

By a News Reporter-Staff News Editor at Agriculture Week -- Researchers detail new data in Enzymes and Coenzymes. According to news reporting from Mexico City, Mexico, by VerticalNews journalists, research stated, "Avocado (Persea americana Mill, cv. Hass) fruit ranks tenth in terms of the most important products for Mexico."

The news correspondents obtained a quote from the research, "Avocado products are quite unstable due to the presence of oxidative enzymes such as polyphenol oxidase and peroxidase. The present study is to characterize the activity of purified avocado peroxidase from avocado in order to ascertain the biochemical and kinetic properties and their inhibition conditions. Purification was performed by Sephacryl S 200 HR gel filtration chromatography and its estimated molecular weight was 40 kDa. The zymogram showed an isoelectric point of 4.7. Six substrates were tested in order to ascertain the affinity of the enzyme for these substrates. The purified peroxidase was found to have low K-m (0.296 mM) and high catalytic efficiency (2688 mM(-1) s(-1)) using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), optimum activity being reached at 51 degrees C, pH 3.8. The addition of dithiothreitol, beta-mercaptoethanol, ascorbic acid, sodium azide, L-cysteine and Tween-20 had high inhibitory effects, while metals ions such as Cu+, Fe2+ andMn(2+) had weak inhibitory activity on purified avocado peroxidase."

According to the news reporters, the research concluded: "The purified avocado peroxidase exhibits high inhibition (K-i = 0.37 mu M) with 1.97 mu M n-propyl gallate using ABTS as substrate at 51 degrees C, pH 3.8 for 10 min."

For more information on this research see: Purification and characterization of peroxidase from avocado (Persea americana Mill, cv. Hass). Journal of the Science of Food and Agriculture, 2014;94(9):1844-1853. Journal of the Science of Food and Agriculture can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of the Science of Food and Agriculture - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0010)

Our news journalists report that additional information may be obtained by contacting J.O. Rojas-Reyes, IPN, Lab Bioenerget CIIDIR Sinaloa, Depto Biotecnol Agr, Mexico City, DF, Mexico. Additional authors for this research include V. Robles-Olvera, O. Carvajal-Zarrabal, C.C. Matinez, K.N. Waliszewski and M.G. Aguilar-Uscanga.

Keywords for this news article include: Mexico City, Peroxidases, Oxidoreductases, Enzymes and Coenzymes, North and Central America

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Agriculture Week


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