Studies Conducted at Prince Songkla University on Lactobacillus Recently Reported (Purification and characterization of bacteriocin produced by oral Lactobacillus paracasei SD1)
By a News Reporter-Staff News Editor at Life Science Weekly -- Researchers detail new data in Gram-Positive Bacteria. According to news originating from Hat Yai, Thailand, by NewsRx correspondents, research stated, "The present study aimed to purify and characterize the antimicrobial protein from Lactobacillus paracasei SD1, which is a strain from the human oral cavity. Antimicrobial activity was obtained from purifying the culture supernatant of L. paracasei SD1."
Our news journalists obtained a quote from the research from Prince Songkla University, "Purification of the active compound was achieved with ammonium sulfate precipitation followed by chloroform and gel filtration chromatography. As revealed by SDS-PAGE, the active fraction was homogeneous, showing a protein with an approximate molecular weight of 25,000 Da. It was confirmed as having a molecular mass of 24,028.2 Da by mass spectrometry. The antimicrobial compound, named 'paracasin SD1', exhibited a broad spectrum against oral pathogens. Paracasin SD1 was stable in a pH range between 3.0 and 8.0 at 100 degrees C for 5 min, and showed resistance to alpha-amylase, catalase, lysozyme and whole saliva. However, its activity was lost after proteinase K and trypsin treatment."
According to the news editors, the research concluded: "The results obtained suggest the possibility of using paracasin SD1 for application in prevention/treatment of oral diseases."
For more information on this research see: Purification and characterization of bacteriocin produced by oral Lactobacillus paracasei SD1. Anaerobe, 2014;27():17-21. Anaerobe can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Anaerobe - www.elsevier.com/wps/product/cws_home/622780)
The news correspondents report that additional information may be obtained from P. Wannun, Prince Songkla University, Fac Dental, Dept. of Prevent Dental, Hat Yai 90112, Thailand. Additional authors for this research include S. Piwat and R. Teanpaisan (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Hat Yai, Thailand, Bacteriocins, Lactobacillus, Lactobacillales, Lactobacillaceae, Bacterial Proteins, Gram-Positive Rods, Gram-Positive Bacteria, Gram-Positive Asporogenous Rods, Pore Forming Cytotoxic Proteins
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