Researchers from Korea Research Institute of Bioscience and Biotechnology Describe Findings in Enzymes and Coenzymes (Toward a generalized and high-throughput enzyme screening system based on artificial genetic circuits)
By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on Enzymes and Coenzymes is now available. According to news reporting from Daejeon, South Korea, by NewsRx journalists, research stated, "Large-scale screening of enzyme libraries is essential for the development of cost-effective biological processes, which will be indispensable for the production of sustainable biobased chemicals. Here, we introduce a genetic circuit termed the Genetic Enzyme Screening System that is highly useful for high-throughput enzyme screening from diverse microbial metagenomes."
The news correspondents obtained a quote from the research from the Korea Research Institute of Bioscience and Biotechnology, "The circuit consists of two AND logics. The first AND logic, the two inputs of which are the target enzyme and its substrate, is responsible for the accumulation of a phenol compound in cell. Then, the phenol compound and its inducible transcription factor, whose activation turns on the expression of a reporter gene, interact in the other logic gate. We confirmed that an individual cell harboring this genetic circuit can present approximately a 100-fold higher cellular fluorescence than the negative control and can be easily quantified by flow cytometry depending on the amounts of phenolic derivatives. The high sensitivity of the genetic circuit enables the rapid discovery of novel enzymes from metagenomic libraries, even for genes that show marginal activities in a host system. The crucial feature of this approach is that this single system can be used to screen a variety of enzymes that produce a phenol compound from respective synthetic phenyl-substrates, including cellulase, lipase, alkaline phosphatase, tyrosine phenol-lyase, and methyl parathion hydrolase."
According to the news reporters, the research concluded: "Consequently, the highly sensitive and quantitative nature of this genetic circuit along with flow cytometry techniques could provide a widely applicable toolkit for discovering and engineering novel enzymes at a single cell level."
For more information on this research see: Toward a generalized and high-throughput enzyme screening system based on artificial genetic circuits. Acs Synthetic Biology, 2014;3(3):163-71. (American Chemical Society - www.acs.org; Acs Synthetic Biology - www.pubs.acs.org/journal/asbcd6)
Our news journalists report that additional information may be obtained by contacting S.L. Choi, Biochemicals and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology , 125 Gwahak-ro, Yuseong-gu, Daejeon, South Korea. Additional authors for this research include E. Rha, S.J. Lee, H. Kim, K. Kwon, Y.S. Jeong, Y.H. Rhee, J.J. Song, H.S. Kim and S.G Lee (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Daejeon, Genetics, Cytometry, South Korea, Enzymes and Coenzymes.
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